Cloned (Comment) | Organism |
---|---|
expression as beta-galactosidase fusion protein in Escherichia coli, the recombinant enzyme shows hemoglobinase activity, the N197 enzyme form might not be the active one, but the C197 form | Schistosoma mansoni |
expression of C197 in Pichia pastoris, expression as beta-galactosidase fusion protein in Escherichia coli, the recombinant enzyme shows hemoglobinase activity, the N197 enzyme form might not be the active one, but the C197 form | Schistosoma mansoni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E64 | slight inhibition at 1 mM | Schistosoma mansoni | |
iodoacetamide | - |
Schistosoma mansoni | |
leupeptin | slight inhibition at 1 mM | Schistosoma mansoni | |
additional information | no inhibition by Z-Phe-Ala-CHN2 and 1,10-phenanthroline | Schistosoma mansoni | |
N-ethylmaleimide | - |
Schistosoma mansoni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted into the digestive tract of host animals | Schistosoma mansoni | - |
- |
extracellular | the enzyme is secreted into the digestive tract of host animals | Schistosoma japonicum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by DTT | Schistosoma mansoni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma japonicum |
32000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation | Schistosoma mansoni |
32000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma mansoni |
50000 | - |
x * 31000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma japonicum |
50000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation | Schistosoma mansoni |
50000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma mansoni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | Schistosoma mansoni | the enzyme is involved in host hemoglobin degradation | ? | - |
? | |
Hemoglobin + H2O | Schistosoma japonicum | the enzyme is involved in host hemoglobin degradation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q99538 | - |
- |
Schistosoma japonicum | P42665 | the enzyme contains a Cys at position 197 | - |
Schistosoma mansoni | P09841 | from infected mice and humans | - |
Schistosoma mansoni | Q9NFY9 | fragment; from infected mice and humans | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | autoactivation | Schistosoma mansoni |
proteolytic modification | autoactivation | Schistosoma japonicum |
proteolytic modification | autoactivation at 37°C, pH 4.5 | Schistosoma mansoni |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity | Schistosoma mansoni |
soluble recombinant C197 from Pichia pastoris, native enzyme to homogeneity | Schistosoma mansoni |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds | active site His151 | Schistosoma mansoni | |
hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds | active site His151 and Cys197 | Schistosoma mansoni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | activity only in adult worms | Schistosoma mansoni | - |
additional information | activity only in adult worms | Schistosoma japonicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Schistosoma mansoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | - |
Schistosoma japonicum | ? | - |
? | |
Hemoglobin + H2O | the enzyme is involved in host hemoglobin degradation | Schistosoma mansoni | ? | - |
? | |
Hemoglobin + H2O | the enzyme is involved in host hemoglobin degradation | Schistosoma japonicum | ? | - |
? | |
Hemoglobin + H2O | the recombinant beta-galactosidase fusion enzyme shows hemoglobinase activity | Schistosoma mansoni | ? | - |
? | |
additional information | the enzyme strictly requires Asn at P1, and prefers Ala, Thr, Val, Asn in descending order at P2, and Thr, Ala, Val, and Ile at P3, overview | Schistosoma mansoni | ? | - |
? | |
Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Schistosoma mansoni | Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
Z-Ala-Pro-Asn-7-amido-4-methylcoumarin + H2O | - |
Schistosoma mansoni | Z-Ala-Pro-Asn + 7-amino-4-methylcoumarin | - |
? | |
Z-Ala-Ser-Asn-7-amido-4-methylcoumarin + H2O | - |
Schistosoma mansoni | Z-Ala-Ser-Asn + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma japonicum |
? | x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation | Schistosoma mansoni |
? | x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma mansoni |
More | structure analysis, overview | Schistosoma mansoni |
More | structure analysis, overview | Schistosoma japonicum |
Synonyms | Comment | Organism |
---|---|---|
Antigen Sj32 | - |
Schistosoma japonicum |
Antigen SM32 | - |
Schistosoma mansoni |
C197 legumain | - |
Schistosoma mansoni |
c197 Sm32 | - |
Schistosoma mansoni |
cysteine protease 1 | - |
Homo sapiens |
hemoglobinase | - |
Schistosoma mansoni |
hemoglobinase | - |
Schistosoma japonicum |
LGMN | - |
Homo sapiens |
More | the enzyme belongs to the C13 peptidase family | Homo sapiens |
More | the enzyme belongs to the C13 peptidase family | Schistosoma mansoni |
More | the enzyme belongs to the C13 peptidase family | Schistosoma japonicum |
N197 legumain | - |
Schistosoma mansoni |
schistosome legumain | - |
Schistosoma mansoni |
schistosome legumain | - |
Schistosoma japonicum |
Sj32 | - |
Schistosoma japonicum |
Sm32 | - |
Schistosoma mansoni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | - |
Schistosoma mansoni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Schistosoma mansoni |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.8 | recombinant enzyme | Schistosoma mansoni |