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Literature summary for 3.4.22.34 extracted from

  • Choi, S.J.; Reddy, S.V.; Devlin, R.D.; Menaa, C.; Chung, H.; Boyce, B.B.; Roodman, G.D.
    Identification of human asparaginyl endopeptidase (legumain) as an inhibitor of osteoclast formation and bone resorption (1999), J. Biol. Chem., 274, 27747-27753.
    View publication on PubMed
Search for more literature for 3.4.22.34

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli BL21, stable expression in 293 cells and secretion to the medium Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE, x * 50000, native wild-type and recombinant enzyme from 293 cells, SDS-PAGE Homo sapiens
50000
-
 x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE, x * 50000, native wild-type and recombinant enzyme from 293 cells, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme inhibits osteoclast-like multinucleated cell formation in humans by more than 60% and bone resorption in the fetal rat long bone ?
-
 ?
protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q99538
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow plasma Homo sapiens
-
additional information tissue distribution, low expression level in all tissues tested, no expression in MG63 osteosarcoma cells Homo sapiens
-
osteoblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme inhibits osteoclast-like multinucleated cell formation in humans by more than 60% and bone resorption in the fetal rat long bone Homo sapiens ?
-
 ?
protein + H2O
-
 Homo sapiens peptides
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE, x * 50000, native wild-type and recombinant enzyme from 293 cells, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
Asparaginyl endopeptidase
-
 Homo sapiens