Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Human cystatin C | competitive inhibition | Sus scrofa | |
human cystatin C mutant L9G | inhibitory potential similar to wild-type cystatin C | Sus scrofa | |
human cystatin C mutant N39K | reduced inhibitory effect compared to the wild-type cystatin C | Sus scrofa | |
human cystatin C mutant R8G | inhibitory potential similar to wild-type cystatin C | Sus scrofa | |
human cystatin C mutant V10G | inhibitory potential similar to wild-type cystatin C | Sus scrofa | |
human cystatin C mutant W106G | inhibitory potential similar to wild-type cystatin C | Sus scrofa | |
human cystatin E/M | type 2 cystatin | Sus scrofa | |
human cystatin F | type 2 cystatin | Sus scrofa | |
additional information | inhibition mechanism by human cystatins, enzyme inhibitor complex formation analysis, no inhibition by human type 1 cystatins A and B, type 2 cystatin D, and type 3 low MW kininogen | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33100 | - |
1 * 33100, glycosylated native enzyme, SDS-PAGE | Sus scrofa |
35300 | - |
glycosylated native enzyme, gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Sus scrofa | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
purified enzyme | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Asn-4-nitroanilide + H2O | - |
Sus scrofa | benzoyl-Asn + 4-nitroaniline | - |
? | |
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Sus scrofa | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
protein + H2O | - |
Sus scrofa | peptides | - |
? | |
protein + H2O | an Asn residue at P1 position is strictly required | Sus scrofa | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33100, glycosylated native enzyme, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
assay at | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000000016 | - |
human cystatin E/M | pH 5.6, 37°C | Sus scrofa | |
0.0000002 | - |
Human cystatin C | pH 5.6, 37°C | Sus scrofa | |
0.00000022 | - |
human cystatin C mutant R8G | pH 5.6, 37°C | Sus scrofa | |
0.00000022 | - |
human cystatin C mutant L9G | pH 5.6, 37°C | Sus scrofa | |
0.00000022 | - |
human cystatin C mutant V10G | pH 5.6, 37°C | Sus scrofa | |
0.00000022 | - |
human cystatin C mutant W106G | pH 5.6, 37°C | Sus scrofa | |
0.00001 | - |
human cystatin F | pH 5.6, 37°C | Sus scrofa | |
0.001 | - |
human cystatin C mutant N39K | above, pH 5.6, 37°C | Sus scrofa |