Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis | Homo sapiens |
partial DNA sequence determination | Sus scrofa |
General Stability | Organism |
---|---|
enzyme tends to be absorbed to any solid material at low salt concentration | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Human cystatin C | - |
Sus scrofa | |
iodoacetamide | - |
Sus scrofa | |
iodoacetate | - |
Sus scrofa | |
additional information | no inhibition by E-64, i.e. trans-epoxysuccinyl-L-leucylamido-(4-guanidino)-butane, no inhibition by PMSF, pepstatin and 1,10-phenanthroline | Sus scrofa | |
N-ethylmaleimide | - |
Sus scrofa | |
N-Phenylmaleimide | - |
Sus scrofa | |
ovocystatin | from chicken egg white | Sus scrofa | |
p-chloromercuribenzoate | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide | pH 5.8, 30°C | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 34000, glycosylated enzyme, SDS-PAGE, x * 31000, deglycosylated enzyme, SDS-PAGE | Sus scrofa |
34000 | - |
x * 34000, glycosylated enzyme, SDS-PAGE, x * 31000, deglycosylated enzyme, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Sus scrofa | - |
peptides | - |
? | |
protein + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q99538 | - |
- |
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 4 potential N-glycosylation sites at Asn91, ASn167, Asn263, and Asn272 | Sus scrofa |
glycoprotein | 4 potential N-glycosylation sites at Asn91, ASn167, Asn263, and Asn272 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
2200fold from kidney, to homogeneity | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
liver | - |
Sus scrofa | - |
additional information | no activity in blood leukocytes | Homo sapiens | - |
placenta | low activity | Homo sapiens | - |
spleen | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.1 | - |
purified enzyme, substrate benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide, pH 5.8, 30°C | Sus scrofa |
Storage Stability | Organism |
---|---|
4°C to -20°C, pH 5.8, 0.05 M sodium citrate, 0.2 M NaCl, 1 mM EDTA, 10 mm cysteine, 0.1% CHAPS, stable for several months with little loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allatotropin + H2O | - |
Sus scrofa | ? | - |
? | |
azocasein + H2O | - |
Sus scrofa | ? | - |
? | |
benzoyl-Asn-4-nitroanilide + H2O | - |
Sus scrofa | benzoyl-Asn + 4-nitroaniline | - |
? | |
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Sus scrofa | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
bombesin + H2O | - |
Sus scrofa | ? | - |
? | |
chicken vasoactive intestinal peptide fragment 16-28 + H2O | - |
Sus scrofa | ? | - |
? | |
human vasoactive intestinal peptide fragment 1-12 + H2O | - |
Sus scrofa | ? | - |
? | |
additional information | no activity with angiotensin I, [Asn1,Val5]angiotensin II, peptides ASTTNYT and GSNKGAIIGLM, substance P, and dynorphin A fragment 1-13, no activity with Asn-4-methylcoumarin-7-amide or Asp-4-methylcoumarin-7-amide | Sus scrofa | ? | - |
? | |
neurotensin + H2O | - |
Sus scrofa | ? | - |
? | |
neurotensin fragment 1-11 + H2O | - |
Sus scrofa | ? | - |
? | |
pig vasoactive intestinal peptide fragment 1-28 + H2O | - |
Sus scrofa | ? | - |
? | |
protein + H2O | - |
Sus scrofa | peptides | - |
? | |
protein + H2O | - |
Homo sapiens | peptides | - |
? | |
recombinant C-fragment of tetanus toxoid + H2O | cleavage of 3 peptide bonds | Sus scrofa | ? | - |
? | |
somatostatin + H2O | - |
Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, glycosylated enzyme, SDS-PAGE, x * 31000, deglycosylated enzyme, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
asparaginyl endopepidase | - |
Sus scrofa |
Asparaginyl endopeptidase | - |
Homo sapiens |
More | belongs to the peptidase family C13 | Sus scrofa |
More | belongs to the peptidase family C13 | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46 | - |
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide | pH 5.8, 30°C | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
at 30°C | Sus scrofa |
6.4 | - |
at 25°C | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | - |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000005 | - |
Human cystatin C | below, pH 5.8, 30°C | Sus scrofa |