Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 11.6% remaining activity | Phaseolus vulgaris | |
iodoacetate | 55.8 remaining activity | Phaseolus vulgaris | |
additional information | no inhibition with EDTA, PMSF, pepstatin A, leupeptin, and E-64 | Phaseolus vulgaris | |
N-ethylmaleimide | 16.6% remaining activity | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38100 | - |
x * 38100, mature enzyme, SDS-PAGE, x * 53400, pre-proenzyme, SDS-PAGE | Phaseolus vulgaris |
53400 | - |
x * 38100, mature enzyme, SDS-PAGE, x * 53400, pre-proenzyme, SDS-PAGE | Phaseolus vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phaseolin + H2O | Phaseolus vulgaris | initiation of phaseolin proteolysis, enyme plays a key role in mobilization of phaseolin during and after kidney bean germination | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | O24325 | legumain-like proteinase LLP | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | but enzyme contains 1 putative N-glycosylation site at N326 | Phaseolus vulgaris |
proteolytic modification | putative cleavage site is N367-E368 | Phaseolus vulgaris |
Purification (Comment) | Organism |
---|---|
44fold | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | germinated, developmental pattern | Phaseolus vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.04 | - |
purified enzyme, substrate casein | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
phaseolin + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
phaseolin + H2O | initiation of phaseolin proteolysis, enyme plays a key role in mobilization of phaseolin during and after kidney bean germination | Phaseolus vulgaris | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38100, mature enzyme, SDS-PAGE, x * 53400, pre-proenzyme, SDS-PAGE | Phaseolus vulgaris |
Synonyms | Comment | Organism |
---|---|---|
asparaginyl-specific cysteine endopeptidase | - |
Phaseolus vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
assay at | Phaseolus vulgaris |