Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | noncompetitive inhibition at pH 3.5, while both K-and V-type activations of bromelain are observed in the presence of 0.5 mM Ca2+ at pH 4.5 and pH 7.5 | Ananas comosus | |
Cu2+ | competitive inhibition at pH 3.5 with 0.6 mM Cu2+, which changes to an uncompetitive inhibition at pH 4.5 and pH 7.5 | Ananas comosus | |
Hg2+ | uncompetitive inhibition at pH 3.5 and pH 4.5 with 0.6 mM Hg2+ | Ananas comosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Ananas comosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | noncompetitive inhibition at pH 3.5, while both K-and V-type activations of bromelain are observed in the presence of 0.5 mM Ca2+ at pH 4.5 and pH 7.5 | Ananas comosus | |
additional information | modulation of fruit bromelain by divalent ions is pH dependent | Ananas comosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ananas comosus | O23791 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 7.23fold from fruits by ammonium sulfate fractionation, dialysis, and anion exchange chromatography | Ananas comosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Ananas comosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2512.44 | - |
purified native enzyme, pH and temperature not specified in the publication | Ananas comosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | - |
Ananas comosus | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, detailed overview | Ananas comosus |