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Literature summary for 3.4.22.33 extracted from

  • Shukor, M.Y.; Masdor, N.; Baharom, N.A.; Jamal, J.A.; Abdullah, M.P.; Shamaan, N.A.; Syed, M.A.
    An inhibitive determination method for heavy metals using bromelain, a cysteine protease (2008), Appl. Biochem. Biotechnol., 144, 283-291.
    View publication on PubMed
Search for more literature for 3.4.22.33

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inhibits casein degradation, complete inhibition Ananas comosus
Hg2+ inhibits casein degradation, complete inhibition Ananas comosus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information poor or no inhibition by ions K+, Ca2+, Mg2+, Mn2+, Co2+, Bo3+, Al3+, Cr2+, Ni2+, Sn2+, Ag+, Fe2+, Cd2+, Zn2+, W3+, Se2+, As3+, and Pb2+, overview Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Ananas comosus
-
fruit bromelain is a mixture of bromelain A and B, the proteolytic enzymes of pineapple fruit Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Ananas comosus ?
-
 ?
benzoyl-L-arginine ethyl ester + H2O artificial substrate Ananas comosus ?
-
 ?
casein + H2O enzyme is assayed using casein as a substrate with Coomassie dye to track completion of hydrolysis of casein, casein is hydrolysed to completion leading to a brown coloured solution, assay method development as a Bradford dye-binding-bromelain-casein system as an assay for heavy metals, based on colour development and its inhibition by Hg2+ and Cu2+ Ananas comosus ?
-
 ?
additional information bromelain is a cysteine protease containing a single thiol group in the active sites Ananas comosus ?
-
 ?
N-benzoyl-L-tyrosine ethyl ester + H2O artificial substrate Ananas comosus ?
-
 ?
Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O artificial substrate Ananas comosus ?
-
 ?

Synonyms

Synonyms Comment Organism
bromelain A
-
 Ananas comosus
bromelain B
-
 Ananas comosus