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Literature summary for 3.4.22.33 extracted from

  • Ota, S.; Muta, E.; Katahira, Y.; Okamoto, Y.
    Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains (1985), J. Biochem., 98, 219-228.
    View publication on PubMed
Search for more literature for 3.4.22.33

Inhibitors

Inhibitors Comment Organism Structure
additional information scarcely inhibited by chicken chystatin Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE Ananas comosus
27000
-
 x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 forms: FBA and FBB Ananas comosus
fractionation Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzoyl-Gly-Gly-Lys + H2O enzyme form FBA is much more active than FBB Ananas comosus ?
-
 ?
Benzyloxycarbonyl-Ala-Leu + H2O enzyme form FBA is much more active than FBB Ananas comosus Benzyloxycarbonyl-Ala + Leu
-
 ?
Benzyloxycarbonyl-Gly-Leu + H2O enzyme form FBA is much more active than FBB Ananas comosus Benzyloxycarbonyl-Gly + Leu
-
 ?
Benzyloxycarbonyl-Gly-Phe + H2O
-
 Ananas comosus Benzyloxycarbonyl-Gly + Phe
-
 ?
Benzyloxycarbonyl-Gly-Tyr + H2O
-
 Ananas comosus Benzyloxycarbonyl-Gly + Tyr
-
 ?
Benzyloxycarbonyl-Gly-Val + H2O enzyme form FBA is much more active than FBB Ananas comosus Benzyloxycarbonyl-Gly + Val
-
 ?
Carbobenzoxy-Gly-Ala + H2O enzyme form FBA is much more active than FBB Ananas comosus Carbobenzoxy-Gly + Ala
-
 ?
additional information specificity overview Ananas comosus ?
-
 ?
Nalpha-Benzoyl-Arg amide + H2O
-
 Ananas comosus Nalpha-Benzoyl-Arg + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 benzoyl-Ala-Leu, benzoyl-Ala-Phe, enzyme form FBA Ananas comosus
3.5 4 benzoyl-Glu-Tyr, benzoyl-Glu-Phe Ananas comosus
4
-
 benzoyl-Ala-Leu, enzyme form FBA Ananas comosus
6.5
-
 benzoyl-Arg amide, enzyme form FBA Ananas comosus