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Literature summary for 3.4.22.32 extracted from

  • Bhattacharya, R.; Bhattacharyya, D.
    Resistance of bromelain to SDS binding (2009), Biochim. Biophys. Acta, 1794, 698-708.
    View publication on PubMed
Search for more literature for 3.4.22.32

Inhibitors

Inhibitors Comment Organism Structure
additional information reversible inactivation of the protease in 10 mM Na-phosphate, pH 7.5 containing 5 mM of Na-tetrathionate, during purification, reactivation by 8 mM of DTT and 4 mM of EDTA in the same buffer at 25°C Ananas comosus
SDS SDS acts as a partial non-competitive inhibitor, 97% inhibition at 100 mM, detailed interaction study and inhibition mechanism, bromelain is partly resistant to SDS binding and denaturation, overview. SDS at submicellar level, below 5 mM, causes conformation change of bromelain leading to a stable entity Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from stem, reversible inactivation of the protease in 10 mM Na-phosphate, pH 7.5 containing 5 mM of Na-tetrathionate, during purification, reactivation by 8 mM of DTT and 4 mM of EDTA in the same buffer at 25°C, followed by gel filtration Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Ananas comosus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Ananas comosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.145
-
 SDS 25°C Ananas comosus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.55
-
 25°C Ananas comosus SDS