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Literature summary for 3.4.22.32 extracted from
- Bromelain enzymes (1976), Methods Enzymol., 45, 475-485.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha2-Macroglobulin | - |
Ananas comosus | |
| Human plasma | - |
Ananas comosus | |
| additional information | alpha1-antitrypsin | Ananas comosus | |
| Rabbit anti-stem bromelain antibodies | - |
Ananas comosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.53 | - |
N-Benzyloxycarbonyl-L-Phe-L-Ser methyl ester | - |
Ananas comosus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
Ananas comosus, determination from sedimentation and diffusion coefficient | Ananas comosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| photosensitized oxidation of stem bromelain in the presence of methylene blue results in partial loss of the enzymatic activity even if the essential sulfhydryl group is protected against oxidation | Ananas comosus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ananas comosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Ananas comosus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzoyl-L-Arg amide + H2O | - |
Ananas comosus | Benzoyl-L-Arg + NH3 | - |
? | |
| Hemoglobin + H2O | denatured | Ananas comosus | Hydrolyzed hemoglobin | - |
? | |
| N-Benzyloxycarbonyl-L-Phe-L-Ser methyl ester + H2O | - |
Ananas comosus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.4 | - |
N-Benzyloxycarbonyl-L-Phe-L-Ser methyl ester | - |
Ananas comosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
denatured hemoglobin, enzyme form F4 | Ananas comosus |
| 5 | 8 | wide plateau, Nalpha-benzoyl-L-Arg amide | Ananas comosus |
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Release 2023.1 (January 2023)
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