Any feedback?
Literature summary for 3.4.22.32 extracted from
- Bromelain enzymes (1970), Methods Enzymol., 19, 273-284.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| Lyophilization causes 27% loss of activity | Ananas comosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not: DFP | Ananas comosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
benzoyl-L-Arg amide | - |
Ananas comosus |  |
| 32 | - |
benzoyl-L-Arg methyl ester | - |
Ananas comosus | |
| 84 | - |
tosyl-L-Lys ethyl ester | - |
Ananas comosus | |
| 170 | - |
benzoyl-L-Arg ethyl ester | - |
Ananas comosus | |
| 210 | - |
Benzoyl-Gly ethyl ester | - |
Ananas comosus | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 20% v/v, 37°C, 20 min, 33% loss of activity | Ananas comosus |
| Methanol | 25% v/v, 25°C, 20 min, stable | Ananas comosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ananas comosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Ananas comosus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay methods | Ananas comosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-Gly ethyl ester + H2O | - |
Ananas comosus | acetyl-Gly + ethanol | - |
? | |
| Benzoyl-DL-Ala ethyl ester + H2O | - |
Ananas comosus | Benzoyl-DL-Ala + ethanol | - |
? | |
| Benzoyl-Gly ethyl ester + H2O | - |
Ananas comosus | Benzoyl-Gly + ethanol | - |
? | |
| Benzoyl-L-Arg amide + H2O | - |
Ananas comosus | Benzoyl-L-Arg + NH3 | - |
? | |
| Benzoyl-L-Arg ethyl ester + H2O | - |
Ananas comosus | Benzoyl-L-Arg + ethanol | - |
? | |
| Benzoyl-L-Arg methyl ester + H2O | - |
Ananas comosus | Benzoyl-L-Arg + methanol | - |
? | |
| casein + H2O | - |
Ananas comosus | hydrolyzed casein | - |
? | |
| Gly ethyl ester + H2O | - |
Ananas comosus | Gly + ethanol | - |
? | |
| L-Phe ethyl ester + H2O | - |
Ananas comosus | L-Phe + ethanol | - |
? | |
| L-Tyr ethyl ester + H2O | - |
Ananas comosus | L-Tyr + ethanol | - |
? | |
| Tosyl-L-Arg methyl ester + H2O | - |
Ananas comosus | Tosyl-L-Arg + methanol | - |
? | |
| tosyl-L-Lys ethyl ester + H2O | - |
Ananas comosus | Tosyl-L-Lys + ethanol | - |
? | |
| Tosyl-L-Lys methyl ester + H2O | - |
Ananas comosus | Tosyl-L-Lys + methanol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
pH 6.1, 20 min, 50% loss of activity | Ananas comosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
benzoyl-L-Arg amide | - |
Ananas comosus | |
| 0.035 | - |
Tosyl-L-Lys methyl ester | - |
Ananas comosus | |
| 0.11 | - |
benzoyl-L-Arg methyl ester | - |
Ananas comosus | |
| 0.36 | - |
Benzoyl-Gly ethyl ester | - |
Ananas comosus | |
| 0.5 | - |
benzoyl-L-Arg ethyl ester | - |
Ananas comosus | |
| 0.55 | - |
acetyl-Gly ethyl ester | - |
Ananas comosus | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | 5°C, 24 h, stable | Ananas comosus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
