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Literature summary for 3.4.22.32 extracted from

  • Rowan, A.D.; Buttle, D.J.
    Pineapple cysteine endopeptidases (1994), Methods Enzymol., 244, 555-568.
    View publication on PubMed
Search for more literature for 3.4.22.32

Inhibitors

Inhibitors Comment Organism Structure
L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane irreversible Ananas comosus
additional information scarcely inhibited by chicken cystatin (amino acid sequence and implications for weak binding of cystatin) Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0154
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Ananas comosus

Purification (Commentary)

Purification (Comment) Organism
-
 Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay methods Ananas comosus

Storage Stability

Storage Stability Organism
-20°C, stored dry, stable Ananas comosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzoyl-Arg-Arg 4-nitrophenyl ester + H2O
-
 Ananas comosus Benzoyl-Arg-Arg + 4-nitrophenol
-
 ?
Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Benzyloxycarbonyl-Arg-Arg 4-nitrophenyl ester + H2O
-
 Ananas comosus Benzyloxycarbonyl-Arg-Arg + 4-nitrophenol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus