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Literature summary for 3.4.22.32 extracted from

  • Rowan, A.D.; Buttle, D.J.; Barrett, A.J.
    The cysteine proteinases of the pineapple plant (1990), Biochem. J., 266, 869-875.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.32

Inhibitors

Inhibitors Comment Organism Structure
additional information scarcely inhibited by chicken cystatin (amino acid sequence and implications for weak binding of cystatin) Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0097
-
 benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
0.0153
-
 Benzyloxycarbonyl-Gly-Phe-citrulline 4-methylcoumarin 7-amide
-
 Ananas comosus
0.0154
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
0.0831
-
 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Ananas comosus fragments of azocasein
-
 ?
Azocoll + H2O
-
 Ananas comosus Hydrolyzed azocoll
-
 ?
Benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus ?
-
 ?
Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Benzyloxycarbonyl-Gly-Phe-citrulline 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus ?
-
 ?
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Fibrin + H2O
-
 Ananas comosus Hydrolyzed fibrin
-
 ?
Hemoglobin + H2O
-
 Ananas comosus Hydrolyzed hemoglobin
-
 ?
Hide powder + H2O
-
 Ananas comosus Hydrolyzed hide powder
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 Benzyloxycarbonyl-Gly-Phe-citrulline 4-methylcoumarin 7-amide
-
 Ananas comosus
0.13
-
 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
0.893
-
 benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
27
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus