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Literature summary for 3.4.22.31 extracted from

  • Napper, A.D.; Bennett, S.P.; Borowski, M.; Holridge, M.B.; Leonard, M.J.C.; Rogers, E.E.; Duan, Y.; Laursen, R.A.; Reinhold, B.; Shames, S.L.
    Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain (1994), Biochem. J., 301, 727-735.
    View publication on PubMedView publication on EuropePMC
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Inhibitors

Inhibitors Comment Organism Structure
L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane i.e. E-64 Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 benzoyl-Phe-Val-Arg 4-nitroanilide
-
 Ananas comosus
11
-
 benzyloxycarbonyl-Arg-Arg 4-nitroanilide
-
 Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23420
-
 Ananas comosus, electrospray mass spectrometry Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?
Benzyloxycarbonyl-Arg-Arg 4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 benzyloxycarbonyl-Arg-Arg 4-nitroanilide
-
 Ananas comosus
10.9
-
 benzoyl-Phe-Val-Arg 4-nitroanilide
-
 Ananas comosus