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Literature summary for 3.4.22.30 extracted from
- Papaya latex enzymes capable of detoxification of gliadin (2009), Amino Acids, 38, 155-165.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | caricain would be suitable for enzyme therapy in gluten intolerance and appears to have synergistic action with porcine intestinal extracts, potential for enzyme therapy in coeliac disease | Carica papaya |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
procaricain, fractions are estimated from plots of Kav against molecular weight using dextran blue, human gamma-globulin, bovine serum albumin and trypsin inhibitor as standards, mass spectrometric analysis of trypsin-digested fractions from chromatography are carried out by liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry in positive mode | Carica papaya |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gliadin + H2O | Carica papaya | - |
fragments of gliadin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | - |
- |
- |
| Carica papaya | P10056 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme is semi-purified with from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration | Carica papaya |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | - |
Carica papaya | - |
| latex | estimated as a fraction of papaya latex | Carica papaya | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gliadin + H2O | - |
Carica papaya | fragments of gliadin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| caricain | - |
Carica papaya |
| Papaya proteinase OMEGA | - |
Carica papaya |
| procaricain | - |
Carica papaya |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
heat stability of caricain up to 85°C | Carica papaya |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
enzyme is dissolved in phosphate buffer and used with a suspension of lysosomes in 0.154 mol/l sodium chloride | Carica papaya |
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Release 2023.1 (January 2023)
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