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Literature summary for 3.4.22.30 extracted from
- The enhancing of a cysteine proteinase activity at acidic pH by protein engineering, the role of glutamic 50 in the enzyme mechanism of caricain (1998), FEBS Lett., 437, 91-96.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of wild-type and mutant enzymes in Escherichia coli BL21(DE3) | Carica papaya |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D158N | site-directed mutagenesis, reduced activity at pH 6.8 | Carica papaya |
| E50A | site-directed mutagenesis, reduced activity at pH 6.8 | Carica papaya |
| E50A/D158N | site-directed mutagenesis, reduced activity at pH 6.8 | Carica papaya |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane | i.e. E-64, irreversible, active site-directed | Carica papaya |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Carica papaya |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein + H2O | Carica papaya | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | P10056 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli, to homogeneity | Carica papaya |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| hydrolysis of proteins with broad specificity for peptide bonds, similar to those of papain and chymopapain | mechanism | Carica papaya |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from inclusion bodies after overexpression in scherichia coli | Carica papaya |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Carica papaya |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein + H2O | - |
Carica papaya | peptides | - |
? | |
| pyr-Glu-Phe-Leu-4-nitroanilide + H2O | - |
Carica papaya | pyr-Glu-Phe-Leu + 4-nitroaniline | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Carica papaya |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Carica papaya |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH profiles of wild-type and mutant enzymes | Carica papaya |
| 6.8 | - |
- |
Carica papaya |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9.5 | - |
Carica papaya |
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Release 2023.1 (January 2023)
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