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Literature summary for 3.4.22.30 extracted from
- Subsite differences between the active centres of papaya peptidase A and papain as revealed by affinity chromatography. Purification of papaya peptidase A by ionic-strength-dependent affinity adsorption on an immobilized peptide inhibitor of papain (1984), Biochem. J., 219, 727-733.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Gly-Gly(O-benzyl)Tyr-Arg | competitive, affinity is dependent on ionic strength and always lower than that of papain | Carica papaya |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester | - |
Carica papaya | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | - |
Carica papaya | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, preparation of the mercury derivative of fully active papaya peptidase A, 50fold molar excess of DTT, 30 days stable | Carica papaya |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O | - |
Carica papaya | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.7 | - |
N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester | - |
Carica papaya | |
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Release 2023.1 (January 2023)
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