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Literature summary for 3.4.22.3 extracted from

  • Kortt, A.A.; Hinds, J.A.; Zerner, B.
    On the specificity and pH dependence of ficin-catalyzed hydrolyses. Some comparisons with bromelain specificity (1974), Biochemistry, 13, 2029-2037.
    View publication on PubMed
Search for more literature for 3.4.22.3

Application

Application Comment Organism
analysis the enzyme should prove generally useful in peptide sequencing Ficus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
0.0059
-
 Nalpha-carbobenzoxy-Ala p-nitrophenyl ester pH 6.0, 3.17% v/v CH3CN, ficin II Ficus sp.
0.0067
-
 p-nitrophenyl N-trans-cinnamoylglycinate ficin III Ficus sp.
0.0076
-
 p-nitrophenyl N-trans-cinnamoylglycinate ficin II Ficus sp.
0.0087
-
 Nalpha-carbobenzoxy-Lys p-nitrophenyl ester pH 6.0, 3.17% v/v CH3CN, ficin II Ficus sp.
0.012
-
 N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester ficin II Ficus sp.
0.0122
-
 Nalpha-carbobenzoxy-Ala p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
0.0134
-
 Nalpha-carbobenzoxy-Gly p-nitrophenyl ester pH 6.0, 3.17% v/v CH3CN, ficin II Ficus sp.
0.0172
-
 Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
0.0183
-
 p-nitrophenyl N-benzyloxycarbonylglycinate ficin III Ficus sp.
0.0194
-
 p-nitrophenyl N-benzyloxycarbonylglycinate ficin II Ficus sp.
0.0214
-
 p-nitrophenyl N-benzoylglycinate ficin III Ficus sp.
0.024
-
 N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester ficin II Ficus sp.
0.025
-
 p-nitrophenyl N-benzoylglycinate ficin II Ficus sp.
0.0258
-
 Nalpha-carbobenzoxy-Ala p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
0.0327
-
 Nalpha-carbobenzoxy-Val p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
0.0377
-
 Nalpha-carbobenzoxy-Phe p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
0.052
-
 Nalpha-carbobenzoxy-Lys p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
0.0598
-
 Nalpha-carbobenzoxy-Gly p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
0.128
-
 N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester ficin II Ficus sp.
0.2
-
 N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester ficin II Ficus sp.
0.309
-
 p-nitrophenyl N-acetylglycinate ficin III Ficus sp.
0.328
-
 p-nitrophenyl N-acetylglycinate ficin II Ficus sp.
1.1
-
 p-nitrophenyl N-formylglycinate ficin III Ficus sp.

Organism

Organism UniProt Comment Textmining
Ficus sp.
-
 ficin II and ficin III
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester + H2O ficin II Ficus sp. N-benzyloxycarbonyl-D-Ala + 4-nitrophenol
-
 ?
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester + H2O ficin II Ficus sp. N-benzyloxycarbonyl-D-Leu + 4-nitrophenol
-
 ?
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester + H2O ficin II Ficus sp. N-benzyloxycarbonyl-D-Phe + 4-nitrophenol
-
 ?
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester + H2O ficin II Ficus sp. N-benzyloxycarbonyl-D-Tyr + 4-nitrophenol
-
 ?
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester + H2O ficin II Ficus sp. Nalpha-carbobenzoxy-Ala + 4-nitrophenol
-
 ?
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O ficin II Ficus sp. Nalpha-carbobenzoxy-Gly + 4-nitrophenol
-
 ?
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester + H2O ficin II Ficus sp. ?
-
 ?
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester + H2O ficin II Ficus sp. Nalpha-carbobenzoxy-Leu + 4-nitrophenol
-
 ?
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester + H2O ficin II Ficus sp. Nalpha-carbobenzoxy-Lys + 4-nitrophenyl
-
 ?
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester + H2O ficin II Ficus sp. Nalpha-carbobenzoxy-Phe + 4-nitrophenol
-
 ?
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester + H2O ficin II Ficus sp. Nalpha-carbobenzoxy-Tyr + 4-nitrophenol
-
 ?
Nalpha-carbobenzoxy-Val p-nitrophenyl ester + H2O ficin II Ficus sp. Nalpha-carbobenzoxy-Val + 4-nitrophenyl
-
 ?
p-nitrophenyl N-acetylglycinate + H2O ficin II and III Ficus sp. p-nitrophenol + N-acetylglycine
-
 ?
p-nitrophenyl N-benzoylglycinate + H2O ficin II and III Ficus sp. p-nitrophenol + N-benzoylglycine
-
 ?
p-nitrophenyl N-benzyloxycarbonylglycinate + H2O ficin II and III Ficus sp. p-nitrophenol + N-benzyloxycarbonylglycine
-
 ?
p-nitrophenyl N-formylglycinate + H2O ficin II and III Ficus sp. p-nitrophenol + N-formylglycine
-
 ?
p-nitrophenyl N-trans-cinnamoylglycinate + H2O ficin II and III Ficus sp. p-nitrophenol + N-trans-cinnamoylglycine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester ficin II Ficus sp.
0.92
-
 N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester ficin II Ficus sp.
1.02
-
 Nalpha-carbobenzoxy-Val p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
1.2
-
 N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester ficin II Ficus sp.
1.85
-
 N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester ficin II Ficus sp.
2.27
-
 Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
2.3
-
 p-nitrophenyl N-acetylglycinate ficin II Ficus sp.
2.5
-
 p-nitrophenyl N-formylglycinate ficin II Ficus sp.
2.83
-
 Nalpha-carbobenzoxy-Phe p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
2.84
-
 p-nitrophenyl N-acetylglycinate ficin III Ficus sp.
4.5
-
 Nalpha-carbobenzoxy-Leu p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
7.45
-
 Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
7.72
-
 p-nitrophenyl N-benzoylglycinate ficin III Ficus sp.
9.25
-
 Nalpha-carbobenzoxy-Gly p-nitrophenyl ester pH 6.0, 3.17% v/v CH3CN, ficin II Ficus sp.
12.4
-
 Nalpha-carbobenzoxy-Gly p-nitrophenyl ester pH 6.0, 3.17% v/v CH3CN, ficin II Ficus sp.
13.3
-
 p-nitrophenyl N-benzyloxycarbonylglycinate ficin III Ficus sp.
29.8
-
 Nalpha-carbobenzoxy-Ala p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
31
-
 Nalpha-carbobenzoxy-Ala p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
35
-
 Nalpha-carbobenzoxy-Lys p-nitrophenyl ester pH 6.0, 3.17% v/v CH3CN, ficin II Ficus sp.
53.6
-
 p-nitrophenyl N-benzoylglycinate ficin II Ficus sp.
55
-
 Nalpha-carbobenzoxy-Lys p-nitrophenyl ester pH 6.36, 23.2% v/v CH3CN, ficin II Ficus sp.
86.4
-
 p-nitrophenyl N-trans-cinnamoylglycinate ficin II Ficus sp.
95.7
-
 p-nitrophenyl N-benzyloxycarbonylglycinate ficin III Ficus sp.
123
-
 p-nitrophenyl N-trans-cinnamoylglycinate ficin III Ficus sp.