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Literature summary for 3.4.22.3 extracted from

  • Azarkan, M.; Matagne, A.; Wattiez, R.; Bolle, L.; Vandenameele, J.; Baeyens-Volant, D.
    Selective and reversible thiol-pegylation, an effective approach for purification and characterization of five fully active ficin (iso)forms from Ficus carica latex (2011), Phytochemistry, 72, 1718-1731.
    View publication on PubMed
Search for more literature for 3.4.22.3

Inhibitors

Inhibitors Comment Organism Structure
E-64 complete inhibition of all five ficin isoforms at 0.1 mM Ficus carica
iodoacetamide complete inhibition of all five ficin isoforms at 5 mM Ficus carica
methylmethanethiol sulfonate complete inhibition of all five ficin isoforms at 3 mM Ficus carica
additional information all five ficin isoforms are not inhibited by pepstatin A, Pafabloc SC, and EDTA Ficus carica
Nalpha-tosyl-L-lysine chloromethyl ketone complete inhibition of all five ficin isoforms at 0.1 mM Ficus carica
Nalpha-tosyl-L-phenylalanine chloromethylketone complete inhibition of all five ficin isoforms at 0.1 mM Ficus carica

Organism

Organism UniProt Comment Textmining
Ficus carica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
SP-Sepharose column chromatography Ficus carica

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Ficus carica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.044
-
 purified isoform ficin A, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica
0.046
-
 purified isoform ficin B, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica
0.073
-
 purified isoform ficin C, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica
0.11
-
 purified isoform ficin D2, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica
0.117
-
 purified isoform ficin D1, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica
0.127
-
 purified isoform ficin A, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica
0.146
-
 purified isoform ficin D1, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica
0.154
-
 purified isoform ficin D2, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica
0.186
-
 purified isoform ficin C, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica
0.204
-
 purified isoform ficin B, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37°C Ficus carica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
-
 Ficus carica Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide + H2O
-
 Ficus carica tert-butyloxycarbonyl-L-Ala-L-Ala-Gly + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * around 24000, native enzyme, SDS-PAGE Ficus carica

Synonyms

Synonyms Comment Organism
ficin A isoform Ficus carica
ficin B isoform Ficus carica
ficin C isoform Ficus carica
ficin D1 isoform Ficus carica
ficin D2 isoform Ficus carica