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Literature summary for 3.4.22.3 extracted from
- Purification, characterization, and solvent-induced thermal stabilization of ficin from Ficus carica (2008), J. Agric. Food Chem., 56, 11417-11423.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | enzyme is used in the food, pharmaceutical, and detergent industries | Ficus carica |
General Stability
| General Stability | Organism |
|---|---|
| the maximum stabilization effect is observed in terms of thermal stabilization by the co-solvent trehalose | Ficus carica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | activity is completely inhibited, cysteine-group specific inhibitor, confirms the participation of the cysteine residue at the active site of the enzyme | Ficus carica |  |
| p-chloromercuribenzoate | activity is completely inhibited by the cysteine-group specific inhibitor | Ficus carica | |
| Sodium tetrathionate | activity is completely inhibited by the cysteine-group specific inhibitor | Ficus carica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23100 | - |
determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry | Ficus carica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | Ficus carica | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ficus carica | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the enzyme contains three disulfide bonds | Ficus carica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| a major ficin from the commercial crude proteinase mixture preparation of Ficus carica is purified by using a SP-Sepharose cation-exchanger column on fast protein liquid chromatography | Ficus carica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Ficus carica | - |
| latex | - |
Ficus carica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6824 | - |
purifed enzyme against casein | Ficus carica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-D,L-arginine-p-nitroanilide + H2O | - |
Ficus carica | 4-nitroaniline + ? | - |
? | |
| casein + H2O | - |
Ficus carica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | enzyme is a single polypeptide chain protein | Ficus carica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ficin | - |
Ficus carica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the apparent denaturation temperature of ficin in the presence of 10, 20, 30, and 40% sorbitol is 75, 77, 81 and 83°C | Ficus carica |
| additional information | - |
the apparent denaturation temperature of ficin in the presence of 10, 20, 30, and 40% sucrose is 74, 77, 80 and 82°C | Ficus carica |
| additional information | - |
the apparent denaturation temperature of ficin in the presence of 10, 20, 30, and 40% xylitol is 74, 76, 79 and 81°C | Ficus carica |
| additional information | - |
the apparent thermal denaturation temperature of ficin is significantly increased from the control value of 72°C in the presence of all co-solvents | Ficus carica |
| 70 | - |
ficin looses 37% of its activity at 70°C after 10 min of incubation, and in the presence of co-solvents, the activity is protected up to 91%, maximum protection is shown by trehalose and followed by sorbitol, sucrose, and xylitol | Ficus carica |
| 70 | - |
ficin looses nearly 50% of its activity after 20 min of incubation | Ficus carica |
| 70 | - |
in the presence of sorbitol, the enzyme retained 82% of its activity after 20 min of incubation | Ficus carica |
| 70 | - |
in the presence of sucrose, the enzyme retained 80% of its activity after 20 min of incubation | Ficus carica |
| 70 | - |
in the presence of trehalose, the enzyme retained 85% of its activity after 20 min and 53% after 60 min of incubation | Ficus carica |
| 70 | - |
in the presence of xylitol, the enzyme retained 78% of its activity after 20 min of incubation | Ficus carica |
| 73 | - |
apparent denaturation temperature of ficin | Ficus carica |
| 85 | - |
the apparent denaturation temperature of ficin shifts in the presence of 40% trehalose | Ficus carica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
in 50 mM sodium phosphate | Ficus carica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | maximum activity is observed between pH 6.5 and 8.5 | Ficus carica |
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