Cloned (Comment) | Organism |
---|---|
various reporter plasmids are transfected into recombinant vaccinia virus-infected BHK cells alone or with the plasmid pGEM3Z/J1, which expresses the swine vesicular disease virus 2A protease, the plasmids are transfected using FuGene6 into cells previously infected with the vaccinia virus vTF7-3, which expresses the T7 RNA polymerase | picornaviridae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
eIF4G + H2O | picornaviridae | - |
fragments of eIF4G | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
picornaviridae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
in the presence of the 2A protease, the Rluc expression from the mutant encephalomyocarditis virus internal ribosome entry site elements is strongly reduced suggesting that the residual translation (seen in the absence of 2A) is occurring principally by a cap-dependent mechanism | picornaviridae |
additional information | - |
monocistronic RNAs containing the avian encephalomyelitis virus IRES elements with mutations in the stem 1 or stem 2 of the pseudoknot are also very efficiently translated when assayed alone, but this expression is essentially eliminated in the presence of 2A protease | picornaviridae |
additional information | - |
when a dicistronic vector containing the Fluc coding sequence upstream of the wild type encephalomyocarditis virus internal ribosome entry site elements is assayed, it is shown that the encephalomyocarditis virus IRES-directed Rluc expression is unaffected by the coexpression of the 2A protease, but the expression of the upstream open reading frame is strongly inhibited | picornaviridae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
eIF4G + H2O | - |
picornaviridae | fragments of eIF4G | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2A protease | - |
picornaviridae |