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Literature summary for 3.4.22.29 extracted from

  • Belsham, G.J.; Nielsen, I.; Normann, P.; Royall, E.; Roberts, L.O.
    Monocistronic mRNAs containing defective hepatitis C virus-like picornavirus internal ribosome entry site elements in their 5 untranslated regions are efficiently translated in cells by a cap-dependent mechanism (2008), RNA, 14, 1671-1680.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.29

Cloned(Commentary)

Cloned (Comment) Organism
various reporter plasmids are transfected into recombinant vaccinia virus-infected BHK cells alone or with the plasmid pGEM3Z/J1, which expresses the swine vesicular disease virus 2A protease, the plasmids are transfected using FuGene6 into cells previously infected with the vaccinia virus vTF7-3, which expresses the T7 RNA polymerase picornaviridae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
eIF4G + H2O picornaviridae
-
 fragments of eIF4G
-
 ?

Organism

Organism UniProt Comment Textmining
picornaviridae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 in the presence of the 2A protease, the Rluc expression from the mutant encephalomyocarditis virus internal ribosome entry site elements is strongly reduced suggesting that the residual translation (seen in the absence of 2A) is occurring principally by a cap-dependent mechanism picornaviridae
additional information
-
 monocistronic RNAs containing the avian encephalomyelitis virus IRES elements with mutations in the stem 1 or stem 2 of the pseudoknot are also very efficiently translated when assayed alone, but this expression is essentially eliminated in the presence of 2A protease picornaviridae
additional information
-
 when a dicistronic vector containing the Fluc coding sequence upstream of the wild type encephalomyocarditis virus internal ribosome entry site elements is assayed, it is shown that the encephalomyocarditis virus IRES-directed Rluc expression is unaffected by the coexpression of the 2A protease, but the expression of the upstream open reading frame is strongly inhibited picornaviridae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eIF4G + H2O
-
 picornaviridae fragments of eIF4G
-
 ?

Synonyms

Synonyms Comment Organism
2A protease
-
 picornaviridae