Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.29 extracted from

  • Morrison, J.M.; Racaniello, V.R.
    Proteinase 2Apro is essential for enterovirus replication in type I interferon-treated cells (2009), J. Virol., 83, 4412-4422.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.29

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant picornaviruses are isolated from HeLa cells transfected with in vitro-synthesized RNA using DEAE-dextran, RNA transcripts are produced from a picornavirus type 1 Mahoney P1M infectious clone, pT7M, or a picornavirus type 2 Lansing (P2L) infectious clone, pT7L picornaviridae

Protein Variants

Protein Variants Comment Organism
V54A a BseRI fragment 4742-6148 is transferred from Se1-3C-02 DNA to pT7M, leads to defects in trans but not cis cleavage picornaviridae
Y88L the BstEII fragment of picornavirus DNA from nucleotide 3240-3930 is replaced with a PCR product containing the appropriate mutation, the growth of picornavirus mutant is similar to that of wild-type virus in untreated cells but is completely inhibited in IFN-alpha-treated cells picornaviridae
Y88S the BstEII fragment of picornavirus DNA from nucleotide 3240-3930 is replaced with a PCR product containing the appropriate mutation, the growth of picornavirus mutant is similar to that of wild-type virus in untreated cells but is completely inhibited in IFN-alpha-treated cells picornaviridae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information picornaviridae 2Apro processes the viral polyprotein, and it cleaves a variety of host proteins, including the translation proteins eIF4GI, eIF4GII and poly(A)-binding protein ?
-
 ?
additional information picornaviridae important cellular substrate for 2Apro is eIF4G, cleavage of this translation protein leads to inhibition of host protein synthesis during picornavirus infection ?
-
 ?
additional information picornaviridae inhibitory role for 2Apro in the most downstream event in interferon signaling, the antiviral activities of interferon-stimulated genes ?
-
 ?
additional information picornaviridae proteinase 2Apro is essential for enterovirus replication in type I interferon-treated cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
picornaviridae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
infected cell HeLa cells are infected picornaviridae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 2Apro processes the viral polyprotein, and it cleaves a variety of host proteins, including the translation proteins eIF4GI, eIF4GII and poly(A)-binding protein picornaviridae ?
-
 ?
additional information important cellular substrate for 2Apro is eIF4G, cleavage of this translation protein leads to inhibition of host protein synthesis during picornavirus infection picornaviridae ?
-
 ?
additional information inhibitory role for 2Apro in the most downstream event in interferon signaling, the antiviral activities of interferon-stimulated genes picornaviridae ?
-
 ?
additional information proteinase 2Apro is essential for enterovirus replication in type I interferon-treated cells picornaviridae ?
-
 ?

Synonyms

Synonyms Comment Organism
2Apro
-
 picornaviridae
proteinase 2A
-
 picornaviridae