Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Human rhinovirus sp. |
Protein Variants | Comment | Organism |
---|---|---|
134Rstop | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
C101S | intramolecular cleavage is reduced to 52% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 20% of that of the wild-type enzyme, cleavage of eIF4G is 75-100% of that of the wild-type enzyme | Human rhinovirus sp. |
C106S | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
C112S | intramolecular cleavage and cleavage of P8-P8' is completely abolished, no detectable cleavage of eIF5G | Human rhinovirus sp. |
C138A | intramolecular cleavage is reduced to 90% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 200% of that of the wild-type enzyme, cleavage of eIF4G is 75-100% of that of the wild-type enzyme | Human rhinovirus sp. |
C52A | intramolecular cleavage and cleavage of P8-P8' is completely abolished, no detectable cleavage of eIF5G | Human rhinovirus sp. |
C54A | intramolecular cleavage and cleavage of P8-P8' is completely abolished, no detectable cleavage of eIF5G | Human rhinovirus sp. |
C61A | intramolecular cleavage is reduced to 54% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 40% of that of the wild-type enzyme, cleavage of eIF4G is 10-25% of that of the wild-type enzyme | Human rhinovirus sp. |
D105N | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
D105T | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
D132R/R134D | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
D132T | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
D35A | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
D35E | intramolecular cleavage is reduced to 16% of the wild-type activity, cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
D35T | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
DELTAG104 | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
DELTAG107/108 | cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
F130L | intramolecular cleavage is completely abolished | Human rhinovirus sp. |
F130S | intramolecular cleavage is completely abolished | Human rhinovirus sp. |
F130V | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
F136V | intramolecular cleavage is reduced to 57% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 25% of that of the wild-type enzyme, cleavage of eIF4G is 10-25% of that of the wild-type enzyme | Human rhinovirus sp. |
G115A | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
G115S | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
G118A | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
G118S | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
G123A | intramolecular cleavage is reduced to 23% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 8% of that of the wild-type enzyme, cleavage of eIF4G is less than 10% of that of the wild-type enzyme | Human rhinovirus sp. |
G123A/G124A | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
G123S | intramolecular cleavage is reduced to 20% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 10% of that of the wild-type enzyme, cleavage of eIF4G is below 10% of that of the wild-type enzyme | Human rhinovirus sp. |
G124A | intramolecular cleavage is reduced to 22% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 9% of that of the wild-type enzyme, cleavage of eIF4G is 10-25% of that of the wild-type enzyme | Human rhinovirus sp. |
G124S | intramolecular cleavage is reduced to 30% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 10% of that of the wild-type enzyme, cleavage of eIF4G is 10-25% of that of the wild-type enzyme | Human rhinovirus sp. |
H114G | intramolecular cleavage and cleavage of P8-P8' is completely abolished, no detectable cleavage of eIF5G | Human rhinovirus sp. |
H114N | mutant enzyme does not contain Zn2+ | Human rhinovirus sp. |
H114N | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
H135stop | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
H18A | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
H18Y | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
K113P/H114A | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
L19S | cleavage of P8-P8' is reduced to 42% of the wild-type activity | Human rhinovirus sp. |
L19S | intramolecular cleavage activity is reduced to 80% of that of the wild-type activity, cleavage of P8-P8' is reduced to 42% of the wild-type activity | Human rhinovirus sp. |
additional information | the presence of an extensive C-terminal helix, in which Asp132, Arg134, Phe130 and Phe136 play important roles, explains why mutations in this region are generally detrimental to proteinase activity.The zinc-binding motif comprises Cys52, Cys54, Cys112 and His114. Exchange of these residues inactivates the enzyme | Human rhinovirus sp. |
N16A | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
R134Q | intramolecular cleavage and cleavage of P8-P8' is completely abolished, no cleavage of eIF4G is detected | Human rhinovirus sp. |
T121Y | intramolecular cleavage and cleavage of P8-P8' is completely abolished | Human rhinovirus sp. |
Y85K | intramolecular cleavage is reduced to 82% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 39% of that of the wild-type enzyme, cleavage of eIF4G is 25-50% of that of the wild-type enzyme | Human rhinovirus sp. |
Y85T | intramolecular cleavage is reduced to 74% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 32% of that of the wild-type enzyme, cleavage of eIF4G is 25-50% of that of the wild-type enzyme | Human rhinovirus sp. |
Y85T | intramolecular cleavage is reduced to 27% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 8% of that of the wild-type enzyme, cleavage of eIF4G is 10-25% of that of the wild-type enzyme | Human rhinovirus sp. |
Y86F | intramolecular cleavage is reduced to 62% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 21% of that of the wild-type enzyme, cleavage of eIF4G is 25-50% of that of the wild-type enzyme | Human rhinovirus sp. |
Y86K | intramolecular cleavage is reduced to 71% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 34% of that of the wild-type enzyme, cleavage of eIF4G is 25-50% of that of the wild-type enzyme | Human rhinovirus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the enzyme contains a tightly bound Zn2+ required for structural integrity | Human rhinovirus sp. | |
Zn2+ | the zinc-binding motif comprises Cys52, Cys54, Cys112 and His114 | Human rhinovirus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human rhinovirus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
eIF4G + H2O | - |
Human rhinovirus sp. | ? | - |
? | |
additional information | residues Gly123, Gly124, Thr121 and Cys101 are proposed to be involved in the architecture of the substrate binding pocket and to provide the correct environment for the catalytic triad of His18, Asp35, and Cys106. Residues Tyr85 and Tyr86 are thought to participate in substrate recognition | Human rhinovirus sp. | ? | - |
? | |
TRPIITTAGPSDMYV + H2O | - |
Human rhinovirus sp. | TRPIITTA + GPSDMYV | - |
? |