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Literature summary for 3.4.22.28 extracted from
- Optimization of soluble expression and purification of recombinant human rhinovirus type-14 3C protease using statistically designed experiments isolation and characterization of the enzyme (2017), Mol. Biotechnol., 59, 407-424 .
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | human rhinovirus (HRV) 3C protease is widely used in recombinant protein production for various applications such as biochemical characterization and structural biology projects to separate recombinant fusion proteins from their affinity tags in order to prevent interference between these tags and the target proteins | Rhinovirus B14 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of soluble GST-tagged enzyme in Escherichia coli strain BL21(DE3), optimization of the expression using response surface methodology | Rhinovirus B14 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhinovirus B14 | - |
HRV-14 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble GST-tagged enzyme by glutathione affinity chromatography from Escherichia coli strain BL21(DE3), method optimization for large-scale purification | Rhinovirus B14 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | HRV 3C protease shows high and unique specificity. It recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro and cleaves between glutamine and glycine | Rhinovirus B14 | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3C protease | - |
Rhinovirus B14 |
| HRV 3C protease | - |
Rhinovirus B14 |
| HRV type-14 3C protease | - |
Rhinovirus B14 |
| human rhinovirus type-14 3C protease | - |
Rhinovirus B14 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 25 | purified recombinant GST-tagged HRV 3C protease is active at both 4°C and 25°C in a variety of buffers | Rhinovirus B14 |
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