Application | Comment | Organism |
---|---|---|
drug development | the conserved key site of the enzyme may serve as attractive target for the design of broad-spectrum antivirals for multiple viruses in the supercluster | Human rhinovirus sp. |
drug development | the conserved key site of the enzyme may serve as attractive target for the design of broad-spectrum antivirals for multiple viruses in the supercluster | Human poliovirus 1 |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme bound to inhibitor GC376, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular replacement | Human poliovirus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S)-2-([N-[(benzyloxy)carbonyl]-L-leucyl]amino)-1-hydroxy-3-(2-oxopyrrolidin-3-yl)propane-1-sulfonic acid | - |
Human poliovirus 1 | |
(2S)-2-([N-[(benzyloxy)carbonyl]-L-leucyl]amino)-1-hydroxy-3-(2-oxopyrrolidin-3-yl)propane-1-sulfonic acid | - |
Human rhinovirus sp. | |
additional information | efficiency of inhibitors in inhibition of virus replication, quantitative realtime PCR expression analysis, overview | Human poliovirus 1 | |
additional information | efficiency of inhibitors in inhibition of virus replication, quantitative realtime PCR expression analysis, overview | Human rhinovirus sp. | |
N2-[(benzyloxy)carbonyl]-N-[(2S)-1-oxo-3-(2-oxopyrrolidin-3-yl)propan-2-yl]-L-leucinamide | - |
Human poliovirus 1 | |
N2-[(benzyloxy)carbonyl]-N-[(2S)-1-oxo-3-(2-oxopyrrolidin-3-yl)propan-2-yl]-L-leucinamide | - |
Human rhinovirus sp. | |
N2-[(benzyloxy)carbonyl]-N-[(2S)-3,4-dioxo-1-(2-oxopyrrolidin-3-yl)-4-(propan-2-ylamino)butan-2-yl]-L-leucinamide | - |
Human poliovirus 1 | |
N2-[(benzyloxy)carbonyl]-N-[(2S)-3,4-dioxo-1-(2-oxopyrrolidin-3-yl)-4-(propan-2-ylamino)butan-2-yl]-L-leucinamide | - |
Human rhinovirus sp. | |
rupintrivir | - |
Human poliovirus 1 | |
rupintrivir | - |
Human rhinovirus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human poliovirus 1 | - |
- |
- |
Human poliovirus 1 Mahoney | - |
- |
- |
Human rhinovirus sp. | - |
HRV, strains 18, 51, and 68 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O | - |
Human poliovirus 1 | Dabcyl-KTSAVLQ + SGFRKME-EDANS | - |
? | |
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O | - |
Human poliovirus 1 Mahoney | Dabcyl-KTSAVLQ + SGFRKME-EDANS | - |
? | |
Edans-DFHLQGP-Dabcyl + H2O | - |
Human rhinovirus sp. | Edans-DFHLQ + GP-Dabcyl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
Human rhinovirus sp. |
3C protease | - |
Human poliovirus 1 |
3C-like protease | - |
Human rhinovirus sp. |
3cLpro | - |
Human rhinovirus sp. |
3Cpro | - |
Human rhinovirus sp. |
3Cpro | - |
Human poliovirus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Human rhinovirus sp. |
37 | - |
assay at | Human poliovirus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Human rhinovirus sp. |
8 | - |
assay at | Human poliovirus 1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0002 | - |
pH 8.0, 37°C, recombinant enzyme | Human rhinovirus sp. | (2S)-2-([N-[(benzyloxy)carbonyl]-L-leucyl]amino)-1-hydroxy-3-(2-oxopyrrolidin-3-yl)propane-1-sulfonic acid | |
0.00034 | - |
pH 8.0, 37°C, recombinant enzyme | Human rhinovirus sp. | rupintrivir | |
0.00055 | - |
pH 8.0, 37°C, recombinant enzyme | Human rhinovirus sp. | N2-[(benzyloxy)carbonyl]-N-[(2S)-3,4-dioxo-1-(2-oxopyrrolidin-3-yl)-4-(propan-2-ylamino)butan-2-yl]-L-leucinamide | |
0.00065 | - |
pH 8.0, 37°C, recombinant enzyme | Human rhinovirus sp. | N2-[(benzyloxy)carbonyl]-N-[(2S)-1-oxo-3-(2-oxopyrrolidin-3-yl)propan-2-yl]-L-leucinamide | |
0.00177 | - |
pH 8.0, 37°C, recombinant enzyme | Human poliovirus 1 | (2S)-2-([N-[(benzyloxy)carbonyl]-L-leucyl]amino)-1-hydroxy-3-(2-oxopyrrolidin-3-yl)propane-1-sulfonic acid | |
0.00183 | - |
pH 8.0, 37°C, recombinant enzyme | Human poliovirus 1 | rupintrivir | |
0.00202 | - |
pH 8.0, 37°C, recombinant enzyme | Human poliovirus 1 | N2-[(benzyloxy)carbonyl]-N-[(2S)-1-oxo-3-(2-oxopyrrolidin-3-yl)propan-2-yl]-L-leucinamide | |
0.00284 | - |
pH 8.0, 37°C, recombinant enzyme | Human poliovirus 1 | N2-[(benzyloxy)carbonyl]-N-[(2S)-3,4-dioxo-1-(2-oxopyrrolidin-3-yl)-4-(propan-2-ylamino)butan-2-yl]-L-leucinamide |