Application | Comment | Organism |
---|---|---|
medicine | enteroviruses use a dual strategy for host translation shutoff, requiring cleavage of poly(A)-binding protein by 3Cpro and of translation iniation factor 4GI by 2Apro, inhibition of endogenous mRNA translation by 3Cpro in HeLa cells is independent of translation iniation factor 4GI or translation iniation factor 4GI cleavage | Enterovirus C |
Cloned (Comment) | Organism |
---|---|
expressed in HeLa cells | Enterovirus C |
expression in BLR pLys DE3 | Enterovirus C |
Protein Variants | Comment | Organism |
---|---|---|
Q537E | cleavage-resistant mutant of poly(A)-binding protein | Enterovirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterovirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Poly(A)-binding protein + H2O | 3Cpro removes the C-terminal domain that interacts with several translation factors, in HeLa cells 3Cpro inhibits translation of endogenous mRNAs and reporter RNA, 3Cpro-mediated translation inhibition can be restored by the addition of exogenous poly(A)-binding protein | Enterovirus C | ? | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
Enterovirus C |
3Cpro | - |
Enterovirus C |