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Literature summary for 3.4.22.28 extracted from

  • Shih, S.R.; Chiang, C.; Chen, T.C.; Wu, C.N.; Hsu, J.T.; Lee, J.C.; Hwang, M.J.; Li, M.L.; Chen, G.W.; Ho, M.S.
    Mutations at KFRDI and VGK domains of enterovirus 71 3C protease affect its RNA binding and proteolytic activities (2004), J. Biomed. Sci., 11, 239-248.
    View publication on PubMed
Search for more literature for 3.4.22.28

Application

Application Comment Organism
drug development 3Cpro as the molecular target to develop anti enterovirus 71 agents enterovirus

Cloned(Commentary)

Cloned (Comment) Organism
into pET-23a and expressed in Escherichia coli BL21 (DE3 pLysS) enterovirus

Protein Variants

Protein Variants Comment Organism
C147S no proteolytic activity in vivo and in vitro enterovirus
D85N possesses proteolytic activity equal to that of wild type enterovirus
G155A markedly reduced RNA binding activity enterovirus
H40D no proteolytic activity in vivo and in vitro enterovirus
I86A no proteolytic activity enterovirus
K156Q markedly reduced RNA binding activity enterovirus
K82Q possesses proteolytic activity equal to that of wild type enterovirus
R84K possesses proteolytic activity equal to that of wild type enterovirus
R84Q possesses proteolytic activity equal to that of wild type enterovirus
V154S markedly reduced RNA binding activity, possesses proteolytic activity equal to that of wild type enterovirus
V154T still binds to viral RNA though with lower efficiency than the wild type, possesses proteolytic activity equal to that of wild type enterovirus

Organism

Organism UniProt Comment Textmining
enterovirus
-
 enterovirus 71, TW2231/98
-

Purification (Commentary)

Purification (Comment) Organism
-
 enterovirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 3Cpro possess RNA-binding activity, the regions responsible for RNA binding are KFRDI (positions 82-86) and VGK (positions 154-156) enterovirus ?
-
 ?
polypeptide translated from plasmid DNA T7-delta3A-3B-3Cmut-delta3D
-
 enterovirus delta3A-3B-3Cmut + 3Cmut-delta3D + 3B-3Cmut-delta3D
-
 ?

Synonyms

Synonyms Comment Organism
3C protease
-
 enterovirus
3Cpro
-
 enterovirus