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Literature summary for 3.4.22.28 extracted from

  • Hata, S.; Sato, T.; Sorimachi, H.; Ishiura, S.; Suzuki, K.
    A simple purification and fluorescent assay method of the poliovirus 3C protease searching for specific inhibitors (2000), J. Virol. Methods, 84, 117-126.
    View publication on PubMed
Search for more literature for 3.4.22.28

Cloned(Commentary)

Cloned (Comment) Organism
a cDNA fragment containing the 3C protease coding region is inserted into pET22b vector and expressed in Escherichia coli Enterovirus C

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl disulfide 0.4 mM, complete inhibition Enterovirus C
Aprotinin 0.1 mM, 64% inhibition Enterovirus C
calpastatin 0.8 mM, 30% inhibition Enterovirus C
chymostatin 0.1 mM, 54% inhibition Enterovirus C
iodoacetic acid 0.4 mM, complete inhibition Enterovirus C
PMSF 1.7 mM, 32% inhibition Enterovirus C
TLCK 0.2 mM, 51% inhibition Enterovirus C
Zn2+ 2.0 mM ZnCl2, complete inhibition Enterovirus C

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, SDS-PAGE Enterovirus C

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poliovirus polyprotein + H2O Enterovirus C the polyprotein is cleaved into mature proteins predominantly by the viral 3C protease hydrolyzed poliovirus polyprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Enterovirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterovirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-yl-MEALFQGPLQY-2,4-dinitrophenyl amide + H2O
-
 Enterovirus C ?
-
 ?
poliovirus polyprotein + H2O the polyprotein is cleaved into mature proteins predominantly by the viral 3C protease Enterovirus C hydrolyzed poliovirus polyprotein
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Enterovirus C