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Literature summary for 3.4.22.28 extracted from

  • Polgar, L.; Erdelyi, F.; Hajnal, E.; Low, M.; Graf, L.; Korant, B.D.
    Separation of native and truncated forms of poliovirus protease 3C produced in Escherichia coli (1993), Biochem. J., 290, 797-800.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM 109 Enterovirus C

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Enterovirus C

Localization

Localization Comment Organism GeneOntology No. Textmining
inclusion body recombinant enzyme, expressed in Escherichia coli Enterovirus C 16234
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
poliovirus, recombinant enzyme, gel filtration Enterovirus C

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
picornavirus polyprotein + H2O Enterovirus C involved in proteolytic processing of virus polyprotein hydrolyzed picornavirus polyprotein
-
?
picornavirus polyprotein + H2O Enterovirus C essential for processing of virus polyprotein containing structural proteins and enzymes including the protease 3C itself hydrolyzed picornavirus polyprotein
-
?
poliovirus polyprotein + H2O Enterovirus C essential for processing of virus polyprotein containing strucutral proteins and enzymes including the protease 3C itself hydrolyzed poliovirus polyprotein
-
?
poliovirus polyprotein + H2O Enterovirus C involved in proteolytic processing of poliovirus polyprotein hydrolyzed poliovirus polyprotein
-
?

Organism

Organism UniProt Comment Textmining
Enterovirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Enterovirus C

Storage Stability

Storage Stability Organism
-80°C, in 0.05 M phosphate buffer, pH 6.8, 10 mM cysteine, 5 mM EDTA, 10% glycerol, stable Enterovirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-Nle-Nle-Nle-Glu-Ala-Leu-Phe-Gln-Gly-Pro-(4-nitro)Phe + H2O synthetic peptide spanning the 2C/3A cleavage region of poliovirus polyprotein Enterovirus C 2-aminobenzoyl-Nle-Nle-Nle-Glu-Ala-Leu-Phe-Gln + Gly-Pro-(4-nitro)Phe
-
?
picornavirus polyprotein + H2O involved in proteolytic processing of virus polyprotein Enterovirus C hydrolyzed picornavirus polyprotein
-
?
picornavirus polyprotein + H2O essential for processing of virus polyprotein containing structural proteins and enzymes including the protease 3C itself Enterovirus C hydrolyzed picornavirus polyprotein
-
?
poliovirus polyprotein + H2O essential for processing of virus polyprotein containing strucutral proteins and enzymes including the protease 3C itself Enterovirus C hydrolyzed poliovirus polyprotein
-
?
poliovirus polyprotein + H2O involved in proteolytic processing of poliovirus polyprotein Enterovirus C hydrolyzed poliovirus polyprotein
-
?

Subunits

Subunits Comment Organism
monomer 1 * 25000, SDS-PAGE Enterovirus C