Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM 109 | Enterovirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Enterovirus C |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inclusion body | recombinant enzyme, expressed in Escherichia coli | Enterovirus C | 16234 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
poliovirus, recombinant enzyme, gel filtration | Enterovirus C |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
picornavirus polyprotein + H2O | Enterovirus C | involved in proteolytic processing of virus polyprotein | hydrolyzed picornavirus polyprotein | - |
? | |
picornavirus polyprotein + H2O | Enterovirus C | essential for processing of virus polyprotein containing structural proteins and enzymes including the protease 3C itself | hydrolyzed picornavirus polyprotein | - |
? | |
poliovirus polyprotein + H2O | Enterovirus C | essential for processing of virus polyprotein containing strucutral proteins and enzymes including the protease 3C itself | hydrolyzed poliovirus polyprotein | - |
? | |
poliovirus polyprotein + H2O | Enterovirus C | involved in proteolytic processing of poliovirus polyprotein | hydrolyzed poliovirus polyprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterovirus C |
Storage Stability | Organism |
---|---|
-80°C, in 0.05 M phosphate buffer, pH 6.8, 10 mM cysteine, 5 mM EDTA, 10% glycerol, stable | Enterovirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-Nle-Nle-Nle-Glu-Ala-Leu-Phe-Gln-Gly-Pro-(4-nitro)Phe + H2O | synthetic peptide spanning the 2C/3A cleavage region of poliovirus polyprotein | Enterovirus C | 2-aminobenzoyl-Nle-Nle-Nle-Glu-Ala-Leu-Phe-Gln + Gly-Pro-(4-nitro)Phe | - |
? | |
picornavirus polyprotein + H2O | involved in proteolytic processing of virus polyprotein | Enterovirus C | hydrolyzed picornavirus polyprotein | - |
? | |
picornavirus polyprotein + H2O | essential for processing of virus polyprotein containing structural proteins and enzymes including the protease 3C itself | Enterovirus C | hydrolyzed picornavirus polyprotein | - |
? | |
poliovirus polyprotein + H2O | essential for processing of virus polyprotein containing strucutral proteins and enzymes including the protease 3C itself | Enterovirus C | hydrolyzed poliovirus polyprotein | - |
? | |
poliovirus polyprotein + H2O | involved in proteolytic processing of poliovirus polyprotein | Enterovirus C | hydrolyzed poliovirus polyprotein | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, SDS-PAGE | Enterovirus C |