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Literature summary for 3.4.22.27 extracted from

  • Kim, N.Y.; Ahn, S.J.; Lee, A.R.; Seo, J.S.; Kim, M.S.; Kim, J.K.; Chung, J.K.; Lee, H.H.
    Cloning, expression analysis and enzymatic characterization of cathepsin S from olive flounder (Paralichthys olivaceus) (2010), Comp. Biochem. Physiol. B, 157, 238-247.
    View publication on PubMed
Search for more literature for 3.4.22.27

Activating Compound

Activating Compound Comment Organism Structure
Brij 35 0.011% slight increases (over 10%) Paralichthys olivaceus
Triton X-100 slight increases (over 10%) Paralichthys olivaceus
Tween 20 0.01%, slight increases (over 10%) Paralichthys olivaceus

Cloned(Commentary)

Cloned (Comment) Organism
the cDNA encoding proenzyme of PoCtS is expressed in Escherichia coli as a fusion protein with glutathione S-transferase in a pGEX-4T-1 vector. The recombinant proPoCtS protein is overexpressed in Escherichia coli BL21(DE3) as a 60 kDa fusion protein Paralichthys olivaceus

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100
-
 Paralichthys olivaceus
Tween 20
-
 Paralichthys olivaceus

Metals/Ions

Metals/Ions Comment Organism Structure
CoCl2 decreased activity Paralichthys olivaceus
HgCl2 decreased activity Paralichthys olivaceus

Organism

Organism UniProt Comment Textmining
Paralichthys olivaceus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information ubiquitous expression pattern Paralichthys olivaceus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Z-Phe-Arg-AMC + H2O
-
 Paralichthys olivaceus ?
-
 ?
Z-Val-Val-Arg-AMC + H2O
-
 Paralichthys olivaceus ?
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin S
-
 Paralichthys olivaceus
PoCtS
-
 Paralichthys olivaceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Paralichthys olivaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Paralichthys olivaceus

Expression

Organism Comment Expression
Paralichthys olivaceus PoCtS expression increased significantly in muscle 6 h post-injection of bacterial lipopolysaccharide up