Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Brij 35 | 0.011% slight increases (over 10%) | Paralichthys olivaceus | |
Triton X-100 | slight increases (over 10%) | Paralichthys olivaceus | |
Tween 20 | 0.01%, slight increases (over 10%) | Paralichthys olivaceus |
Cloned (Comment) | Organism |
---|---|
the cDNA encoding proenzyme of PoCtS is expressed in Escherichia coli as a fusion protein with glutathione S-transferase in a pGEX-4T-1 vector. The recombinant proPoCtS protein is overexpressed in Escherichia coli BL21(DE3) as a 60 kDa fusion protein | Paralichthys olivaceus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | - |
Paralichthys olivaceus | |
Tween 20 | - |
Paralichthys olivaceus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | decreased activity | Paralichthys olivaceus | |
HgCl2 | decreased activity | Paralichthys olivaceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paralichthys olivaceus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | ubiquitous expression pattern | Paralichthys olivaceus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Z-Phe-Arg-AMC + H2O | - |
Paralichthys olivaceus | ? | - |
? | |
Z-Val-Val-Arg-AMC + H2O | - |
Paralichthys olivaceus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cathepsin S | - |
Paralichthys olivaceus |
PoCtS | - |
Paralichthys olivaceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Paralichthys olivaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paralichthys olivaceus |
Organism | Comment | Expression |
---|---|---|
Paralichthys olivaceus | PoCtS expression increased significantly in muscle 6 h post-injection of bacterial lipopolysaccharide | up |