Literature summary for 3.4.22.27 extracted from

Kramer, G.; Paul, A.; Kreusch, A.; Schueler, S.; Wiederanders, B.; Schilling, K.
Optimized folding and activation of recombinant procathepsin L and S produced in Escherichia coli (2007), Protein Expr. Purif., 54, 147-156.

Search for more literature for 3.4.22.27

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
E-64	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0184	-	benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin	at pH 6.5 and 37°C	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24560	-	calculated from amino acid sequence	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P25774	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and phenyl-Sepharose column chromatography	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
folding is initiated by 100fold dilution of denatured-reduced proenzymes into folding buffers, generally containing 1-2 mM EDTA, most effective in promotion of procathepsin S folding is arginine (1 M)	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
26	-	at temperatures above 26°C activity rapidly declines after short initial increase	Homo sapiens

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4.6	-	best condition for auto-activation	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
42	-	at pH 6.5 and 37°C	Homo sapiens	benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin

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Release 2023.1 (January 2023)