Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inclusion body | recombinant enzyme expressed in Escherichia coli | Homo sapiens | 16234 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the proenzyme is autocatalytically processed to active cathepsin S at pH 4.5 in the presence of an excess of cysteine and catalytic amounts of dextran sulfate | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
recombinant procathepsin S from inclusion bodies produced in Escherichia coli is renatured at pH 7.6 by a two-step dilution | Homo sapiens |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Homo sapiens |
More | most of the loss of procathepsin S occurs during folding, probably because of aggregation. Concentrations lower than 0.02 mg/ml of procathepsin S are necessary to minimise such aggregation | Homo sapiens |