Literature summary for 3.4.22.27 extracted from

Br�mme, D.; Steinert, A.; Friebe, S.; Fittkau, S.; Wiederanders, B.; Kirschke, H.
The specificity of bovine spleen cathepsin S. A comparison with rat liver cathepsins L and B (1989), Biochem. J., 264, 475-481.

Search for more literature for 3.4.22.27

Inhibitors

Inhibitors	Comment	Organism	Structure
benzyloxycarbonyl Phe-Ala-CH2F	very potent time-dependent inhibitor, inhibits proteinase 30times more efficiently than it does cathepsin L and about 300times better than it does cathepsin B	Bos taurus
benzyloxycarbonyl-Phe-Ala-CH2F	-	Bos taurus
benzyloxycarbonyl-Phe-Lys(benzyloxycarbonyl)-CH3	inhibition in the micromolar range, competitive	Bos taurus
benzyloxycarbonyl-Val-Val-Phe-CH3	inhibition in the micromolar range, competitive	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0073	-	tert-butyloxycarbonyl-Phe-Leu-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus
0.0081	-	benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus
0.0147	-	benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus
0.0175	-	benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus
0.0375	-	tert-butyloxycarbonyl-Phe-Phe-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
spleen	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O	-	Bos taurus	benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O	-	Bos taurus	benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Val-Val-7-amido-4-methylcoumarin + H2O	-	Bos taurus	benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O	-	Bos taurus	benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O	-	Bos taurus	benzyloxycarbonyl-L-Val-L-Val-L-Arg + 7-amino-4-methylcoumarin	-	?
additional information	the enzyme prefers small neutral amino acid residues in the subsites S2 and S3	Bos taurus	?	-	?
Oxidized insulin B-chain + H2O	major cleavage sites: Glu13-Ala14, Leu17-Val18 and Phe25-Tyr26. Minor cleavage sites: Asn3-Gln4, Ser9-His10 and Leu15-Tyr16	Bos taurus	?	-	?
tert-butyloxycarbonyl-Phe-Leu-Arg-7-amido-4-methylcoumarin + H2O	-	Bos taurus	tert-butyloxycarbonyl-Phe-Leu-Arg + 7-amino-4-methylcoumarin	-	?
tert-butyloxycarbonyl-Phe-Phe-Arg-7-amido-4-methylcoumarin + H2O	-	Bos taurus	tert-butyloxycarbonyl-Phe-Phe-Arg + 7-amino-4-methylcoumarin	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.7	-	benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus
6.1	-	tert-butyloxycarbonyl-Phe-Leu-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus
8.6	-	tert-butyloxycarbonyl-Phe-Phe-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus
13	-	benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus
40.5	-	benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin	pH 6.5, 25°C	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	6.5	reaction with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin	Bos taurus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	8	pH 4.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000018	-	benzyloxycarbonyl-Phe-Ala-CH2F	pH 6.5	Bos taurus
0.0014	-	benzyloxycarbonyl-Phe-Lys(benzyloxycarbonyl)-CH3	pH 6.5	Bos taurus
0.0018	-	benzyloxycarbonyl-Val-Val-CH3	pH 6.5	Bos taurus

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Release 2023.1 (January 2023)