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Literature summary for 3.4.22.27 extracted from

  • Schmid, H.; Koop, M.; Utermann, S.; Lambacher, L.; Mayer, P.; Schaefer, L.
    Specific catalytic activity of cathepsin S in comparison to cathepsins B and L along the rat nephron (1997), Biol. Chem., 378, 61-69.
    View publication on PubMed
Search for more literature for 3.4.22.27

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.288
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus primary role of the enzyme in heterophagocytosis of proteins from the ultrafiltrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney in nephron segments microdissected from lyophilized renal sections, highest cathepsin S activity is found in the proximal convoluted tubules followed by proximal straight tubules of the superficial cortex. In the remaining segments cathepsin S activity is hardly detectable Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O 73% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Rattus norvegicus benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information primary role of the enzyme in heterophagocytosis of proteins from the ultrafiltrate Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 pH 7.5, 60 min, stable Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 reaction with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin or benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: about 20% of maximal activity, pH 7.5: about 40% of maximal activity, reaction with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin or benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 37°C, 60 min, stable Rattus norvegicus