Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antiCP polyclonal antibody | - |
Rattus norvegicus | |
antiFVIIa polyclonal antibody | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | cancer procoagulant is a cysteine protease acting as an activator of coagulation factor X, the protein activates factor X in the absence of tissue factor and factor VII | ? | - |
? | |
additional information | Rattus norvegicus | rat and human cancer procoagulant activity bind similarly to antiCP antibodies | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Lobund-Wistar rat with transplanted PA-3 prostate tumors | - |
Purification (Comment) | Organism |
---|---|
harvested PA3 tumors are extracted in 0.02 M Tris (pH 7.8), after centrifugation extracts are purified by ion-exchange chromatography, fractions containing CP activity are desalted and concentrated by ultrafiltration on YM10 membrane and redissolved in 2-3 ml of 0.02 M Tris | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
prostate gland cancer cell | prostate tumor | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme has procoagulant activity | Rattus norvegicus |
0.059 | - |
crude extract from independent PA3 tumor, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
0.068 | - |
crude extract from independent PA3 prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
0.078 | - |
crude extract from independent PA3 prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
0.082 | - |
crude extract from independent PA3 rat prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
0.098 | - |
crude extract from independent PA3 prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
2.26 | - |
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 33fold purification, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
2.32 | - |
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 30fold purification, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
4.91 | - |
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 50fold purification, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
7.7 | - |
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 133fold purification, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
16.6 | - |
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 202fold purification, activity of samples is determined by using a 3-stage chromogenic assay | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cancer procoagulant is a cysteine protease acting as an activator of coagulation factor X, the protein activates factor X in the absence of tissue factor and factor VII | Rattus norvegicus | ? | - |
? | |
additional information | rat and human cancer procoagulant activity bind similarly to antiCP antibodies | Rattus norvegicus | ? | - |
? | |
Sar-Pro-Arg-p-nitroanilide + H2O | - |
Rattus norvegicus | Sar-Pro-Arg + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cancer procoagulant | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
cancer procoagulant activity from 4 independent prostate tumors is assayed, inhibition of FX-activating activity of cancer procoagulant-containing extracts by use of a polyclonal antiFVIIa antibody, samples obtained from 1-step purification of crude extract are tested for the presence of tissue factor or FVIIa activity in inhibition assay using antirat factor VIIa polyclonal antibodies, 0% inhibition of cancer procoagulant sample 1, 0.4% inhibition of cancer procoagulant sample 2, 6.6% inhibition of cancer procoagulant sample 3, 4.9% inhibition of cancer procoagulant sample 4 | Rattus norvegicus | additional information |