Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.26 extracted from

  • Kamocka, M.; Pollard, M.; Suckow, M.; Mielicki, W.P.; Rosen, E.D.
    Rat prostate tumors express cancer procoagulant, an activator of coagulation factor X (2008), Comp. Med., 58, 282-286.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
antiCP polyclonal antibody
-
Rattus norvegicus
antiFVIIa polyclonal antibody
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus cancer procoagulant is a cysteine protease acting as an activator of coagulation factor X, the protein activates factor X in the absence of tissue factor and factor VII ?
-
?
additional information Rattus norvegicus rat and human cancer procoagulant activity bind similarly to antiCP antibodies ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Lobund-Wistar rat with transplanted PA-3 prostate tumors
-

Purification (Commentary)

Purification (Comment) Organism
harvested PA3 tumors are extracted in 0.02 M Tris (pH 7.8), after centrifugation extracts are purified by ion-exchange chromatography, fractions containing CP activity are desalted and concentrated by ultrafiltration on YM10 membrane and redissolved in 2-3 ml of 0.02 M Tris Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
prostate gland cancer cell prostate tumor Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme has procoagulant activity Rattus norvegicus
0.059
-
crude extract from independent PA3 tumor, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus
0.068
-
crude extract from independent PA3 prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus
0.078
-
crude extract from independent PA3 prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus
0.082
-
crude extract from independent PA3 rat prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus
0.098
-
crude extract from independent PA3 prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus
2.26
-
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 33fold purification, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus
2.32
-
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 30fold purification, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus
4.91
-
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 50fold purification, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus
7.7
-
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 133fold purification, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus
16.6
-
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 202fold purification, activity of samples is determined by using a 3-stage chromogenic assay Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cancer procoagulant is a cysteine protease acting as an activator of coagulation factor X, the protein activates factor X in the absence of tissue factor and factor VII Rattus norvegicus ?
-
?
additional information rat and human cancer procoagulant activity bind similarly to antiCP antibodies Rattus norvegicus ?
-
?
Sar-Pro-Arg-p-nitroanilide + H2O
-
Rattus norvegicus Sar-Pro-Arg + p-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
cancer procoagulant
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
cancer procoagulant activity from 4 independent prostate tumors is assayed, inhibition of FX-activating activity of cancer procoagulant-containing extracts by use of a polyclonal antiFVIIa antibody, samples obtained from 1-step purification of crude extract are tested for the presence of tissue factor or FVIIa activity in inhibition assay using antirat factor VIIa polyclonal antibodies, 0% inhibition of cancer procoagulant sample 1, 0.4% inhibition of cancer procoagulant sample 2, 6.6% inhibition of cancer procoagulant sample 3, 4.9% inhibition of cancer procoagulant sample 4 Rattus norvegicus additional information