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Literature summary for 3.4.22.26 extracted from

  • Mielicki, W.P.; Mielicka, E.; Gordon, S.G.
    Cancer procoagulant activity studies using synthetic peptidyl substrates (1997), Thromb. Res., 87, 251-256.
    View publication on PubMed
Search for more literature for 3.4.22.26

Application

Application Comment Organism
medicine enzyme has potential as a sensitive and early tumor marker Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
 Homo sapiens
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane ie.e. E-64 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 D-Ile-Pro-Arg-4-nitroanilide pH 7.2 Homo sapiens
0.061
-
 N-p-tosyl-Gly-Pro-Arg-4-nitroanilide pH 7.2 Homo sapiens
0.109
-
 D-Ala-Pro-Arg-4-nitroanilide pH 7.2 Homo sapiens
0.153
-
 N-p-tosyl-Gly-Pro-Lys-4-nitroanilide pH 7.2 Homo sapiens
0.306
-
 Glu-Glu-Pro-Arg-4-nitroanilide pH 7.2 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor X + H2O Homo sapiens cleavage of Arg-Ile bond, activation of the coaglutation factor X factor Xa
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
amnion
-
 Homo sapiens
-
chorion
-
 Homo sapiens
-
additional information produced by malignant and fetal cells Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
47
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Leu-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Leu-Pro-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Val-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Val-Pro-Arg + 4-nitroaniline
-
 ?
D-Ala-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens D-Ala-Pro-Arg + 4-nitroaniline
-
 ?
D-Ile-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens D-Ile-Pro-Arg + 4-nitroaniline
-
 ?
D-Phe-Pip-Arg-4-nitroanilide + H2O
-
 Homo sapiens D-Phe-Pip-Arg + 4-nitroaniline
-
 ?
factor X + H2O cleavage of Arg-Ile bond Homo sapiens factor Xa
-
 ?
factor X + H2O cleavage of Arg-Ile bond, activation of the coaglutation factor X Homo sapiens factor Xa
-
 ?
Fibrinogen + H2O
-
 Homo sapiens ?
-
 ?
Glu-Glu-Phe-Lys-4-nitroanilide + H2O
-
 Homo sapiens Glu-Glu-Phe-Lys + 4-nitroaniline
-
 ?
Glu-Glu-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens Glu-Glu-Pro-Arg + 4-nitroaniline
-
 ?
Leu-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens Leu-Pro-Arg + 4-nitroaniline
-
 ?
methylsulfonyl-D-cyclohexylalanyl-butyl-Arg-4-nitroanilide + H2O
-
 Homo sapiens methylsulfonyl-D-cyclohexylalanyl-butyl-Arg + 4-nitroaniline
-
 ?
additional information substrate specificity, overview Homo sapiens ?
-
 ?
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens N-p-tosyl-Gly-Pro-Arg + 4-nitroaniline
-
 ?
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
-
 Homo sapiens N-p-tosyl-Gly-Pro-Lys + 4-nitroaniline
-
 ?
sarcosyl-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens sarcosyl-Pro-Arg + 4-nitroaniline
-
 ?
Val-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens Val-Pro-Arg + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
cancer procoagulant
-
 Homo sapiens
CP
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0073
-
 N-p-tosyl-Gly-Pro-Arg-4-nitroanilide pH 7.2 Homo sapiens
0.0082
-
 D-Ile-Pro-Arg-4-nitroanilide pH 7.2 Homo sapiens
0.0119
-
 Glu-Glu-Pro-Arg-4-nitroanilide pH 7.2 Homo sapiens
0.0129
-
 N-p-tosyl-Gly-Pro-Lys-4-nitroanilide pH 7.2 Homo sapiens
0.0132
-
 D-Ala-Pro-Arg-4-nitroanilide pH 7.2 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens