BRENDA - Enzyme Database
show all sequences of 3.4.22.25

Glycyl endopeptidase

Buttle, D.J.; Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds) 2, 1132-1134 (2004)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
the enzyme depends on a thiolate ion for activity
Carica papaya
Engineering
Protein Variants
Commentary
Organism
G27E
site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position
Carica papaya
G73R
site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position, decreased affinity for cystatin C
Carica papaya
Inhibitors
Inhibitors
Commentary
Organism
Structure
Alpha-macroglobulin
-
Carica papaya
E64
inactivation
Carica papaya
iodoacetamide
slow inactivation
Carica papaya
iodoacetate
slow inactivation
Carica papaya
additional information
no inhibition by most cystatins
Carica papaya
papaya cystatin
-
Carica papaya
Peptidyl diazomethanes
dependent on the substrate
Carica papaya
Pro-peptide
the enzyme is inhibited by its own pro region after cleavage, also pro-peptide of other C1 peptidase family enzymes can be inhibitory
Carica papaya
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
latex
Carica papaya
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23313
-
1 * 23313
Carica papaya
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Carica papaya
the enzyme is useful for the plant defense system
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Carica papaya
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
no glycoprotein
mature enzyme
Carica papaya
proteolytic modification
the enzyme is synthesized as preproenzyme
Carica papaya
Purification (Commentary)
Purification (Commentary)
Organism
different methods, overview
Carica papaya
Source Tissue
Source Tissue
Commentary
Organism
Textmining
latex
-
Carica papaya
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Chicken cystatin + H2O
-
668767
Carica papaya
?
-
-
-
?
Gly-4-nitroanilide + H2O
-
668767
Carica papaya
glycine + 4-nitroaniline
-
-
-
?
Human cystatin C + H2O
-
668767
Carica papaya
?
-
-
-
?
additional information
the enzyme is useful for the plant defense system
668767
Carica papaya
?
-
-
-
-
additional information
substrate specificity with preference for glycyl residues, structure-function relationship, overview, hydrolyzes glycine esters but not Bz-Arg-4-nitroanilide
668767
Carica papaya
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 23313
Carica papaya
More
three-dimensional structure comparison to other latex peptidase
Carica papaya
Synonyms
Synonyms
Commentary
Organism
Chymopapain M
-
Carica papaya
Glycyl endopeptidase
-
Carica papaya
More
the enzyme belongs to the C1A peptidase family
Carica papaya
Papaya peptidase B
-
Carica papaya
Papaya proteinase IV
-
Carica papaya
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
about
Carica papaya
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0000034
-
papapya cystatin
-
Carica papaya
0.00086
-
Pro-peptide
-
Carica papaya
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
the enzyme depends on a thiolate ion for activity
Carica papaya
Engineering (protein specific)
Protein Variants
Commentary
Organism
G27E
site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position
Carica papaya
G73R
site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position, decreased affinity for cystatin C
Carica papaya
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Alpha-macroglobulin
-
Carica papaya
E64
inactivation
Carica papaya
iodoacetamide
slow inactivation
Carica papaya
iodoacetate
slow inactivation
Carica papaya
additional information
no inhibition by most cystatins
Carica papaya
papaya cystatin
-
Carica papaya
Peptidyl diazomethanes
dependent on the substrate
Carica papaya
Pro-peptide
the enzyme is inhibited by its own pro region after cleavage, also pro-peptide of other C1 peptidase family enzymes can be inhibitory
Carica papaya
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0000034
-
papapya cystatin
-
Carica papaya
0.00086
-
Pro-peptide
-
Carica papaya
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
latex
Carica papaya
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23313
-
1 * 23313
Carica papaya
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Carica papaya
the enzyme is useful for the plant defense system
?
-
-
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
no glycoprotein
mature enzyme
Carica papaya
proteolytic modification
the enzyme is synthesized as preproenzyme
Carica papaya
Purification (Commentary) (protein specific)
Commentary
Organism
different methods, overview
Carica papaya
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
latex
-
Carica papaya
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Chicken cystatin + H2O
-
668767
Carica papaya
?
-
-
-
?
Gly-4-nitroanilide + H2O
-
668767
Carica papaya
glycine + 4-nitroaniline
-
-
-
?
Human cystatin C + H2O
-
668767
Carica papaya
?
-
-
-
?
additional information
the enzyme is useful for the plant defense system
668767
Carica papaya
?
-
-
-
-
additional information
substrate specificity with preference for glycyl residues, structure-function relationship, overview, hydrolyzes glycine esters but not Bz-Arg-4-nitroanilide
668767
Carica papaya
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 23313
Carica papaya
More
three-dimensional structure comparison to other latex peptidase
Carica papaya
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
about
Carica papaya
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
753097
Liu
Papain-like cysteine protease ...
Carica papaya
BMC Genomics
19
26
2018
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4
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2
2
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733902
Karnjanapratum
Glycyl endopeptidase from papa ...
Carica papaya
Food Chem.
165
403-411
2014
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1
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1
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679571
Chaiwut
-
Solid-to-solid peptide synthes ...
Carica papaya
Enzyme Microb. Technol.
40
954-960
2007
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6
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6
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667431
Huet
Structural characterization of ...
Carica papaya
Biochem. Biophys. Res. Commun.
341
620-626
2006
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668767
Buttle
-
Glycyl endopeptidase ...
Carica papaya
Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds)
2
1132-1134
2004
1
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652619
Azarkan
Fractionation and purification ...
Carica papaya
J. Chromatogr. B
790
229-238
2003
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651248
Visal
The proregion of papaya protei ...
Carica papaya
FEBS Lett.
434
401-405
1998
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1
1
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5
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2
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1
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1
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30148
Dekeyser
Kinetic constants for the hydr ...
Carica papaya
Arch. Biochem. Biophys.
320
375-379
1995
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30150
O'Hara
Crystal structure of glycyl en ...
Carica papaya
Biochemistry
34
13190-13195
1995
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30151
Thomas
The structural origins of the ...
Carica papaya
Biochem. J.
306
39-46
1995
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30155
Taylor
Expression of the pro-regions ...
Carica papaya
Biochem. Soc. Trans.
23
80S
1995
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30146
Buttle
Glycyl endopeptidase ...
Carica papaya
Methods Enzymol.
244
539-555
1994
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4
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30153
Thomas
Structure of chymopapain M the ...
Carica papaya
Biochem. J.
300
805-820
1994
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30147
Sumner
Factors effecting the thermost ...
Carica papaya
Eur. J. Biochem.
214
129-134
1993
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30144
Buttle
Selective cleavage of glycyl b ...
Carica papaya
FEBS Lett.
260
195-197
1990
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30145
Buttle
Interactions of papaya protein ...
Carica papaya
FEBS Lett.
262
58-60
1990
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30152
Topham
Three-dimensional structure an ...
Carica papaya
Biochem. Soc. Trans.
18
934-935
1990
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30143
Ritonja
Papaya proteinase IV amino aci ...
Carica papaya
FEBS Lett.
258
109-112
1989
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30149
Buttle
Affinity purification of the n ...
Carica papaya
Biochem. J.
261
469-476
1989
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1
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3
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5
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1
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30154
Goodenough
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Chromatographic and electropho ...
Carica papaya
Phytochemistry
26
75-79
1987
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1
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1
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1
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30142
Polgar
Isolation of highly active pap ...
Carica papaya
Biochim. Biophys. Acta
658
262-269
1981
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