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Literature summary for 3.4.22.2 extracted from
- Comparison of the kinetics and mechanism of the papain-catalyzed hydrolysis of esters and thiono esters (1985), Biochemistry, 24, 6808-6818.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
N-(beta-phenylpropionyl)Gly methyl thiono ester | - |
Carica papaya |  |
| 0.3 | - |
benzoyl-thiocarbamic acid methyl ester | - |
Carica papaya | |
| 0.301 | - |
N-benzoyl-Gly methyl thiono ester | - |
Carica papaya | |
| 0.77 | - |
benzoyl-thiocarbamic acid | - |
Carica papaya | |
| 6.63 | - |
N-(beta-phenylpropionyl)Gly methyl ester | - |
Carica papaya | |
| 16 | - |
benzoyl-thiocarbamic acid ethyl ester | - |
Carica papaya | |
| 18.54 | - |
Nalpha-benzoyl-Gly methyl ester | - |
Carica papaya | |
| 21.8 | - |
N-benzoyl-Gly methyl ester | - |
Carica papaya | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-thiocarbamic acid + H2O | - |
Carica papaya | N-benzoyl-Gly + SH2 | - |
? | |
| benzoyl-thiocarbamic acid ethyl ester + H2O | - |
Carica papaya | N-benzoyl-Gly + ethanethiol | - |
? | |
| benzoyl-thiocarbamic acid methyl ester + H2O | - |
Carica papaya | N-benzoyl thioglycine + methanol | - |
? | |
| N(beta-phenylpropionyl)Gly methyl ester + H2O | - |
Carica papaya | ? | - |
? | |
| N-(beta-phenylpropionyl)Gly methyl thiono ester + H2O | - |
Carica papaya | ? | - |
? | |
| N-benzoyl-Gly ethyl ester + H2O | - |
Carica papaya | N-benzoyl-Gly + ethanol | - |
? | |
| N-benzoyl-Gly methyl ester + H2O | - |
Carica papaya | ? | - |
? | |
| N-benzoyl-Gly methyl ester + H2O | - |
Carica papaya | N-benzoyl-Gly + methanol | - |
? | |
| N-benzoyl-Gly methyl thiono ester + H2O | - |
Carica papaya | ? | - |
? | |
| Nalpha-benzoyl-Gly methyl ester + H2O | - |
Carica papaya | Nalpha-benzoyl-Gly + methanol | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.064 | - |
benzoyl-thiocarbamic acid methyl ester | - |
Carica papaya | |
| 3.4 | - |
benzoyl-thiocarbamic acid ethyl ester | - |
Carica papaya | |
| 3.48 | - |
N-benzoyl-Gly methyl ester | - |
Carica papaya | |
| 3340 | - |
benzoyl-thiocarbamic acid | - |
Carica papaya | |
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