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Literature summary for 3.4.22.2 extracted from
- Enhanced activity and stability of papain immobilized on CNBr-activated sepharose (2015), Int. J. Biol. Macromol., 75, 373-377 .
General Stability
| General Stability | Organism |
|---|---|
| immobilization of papain by covalent attachment on Sepharose 6B activated by using cyanogen bromide brings about significant enhancement of storage and thermal stability and of stability at extreme pHs | Carica papaya |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 5 mM, 24% loss of activity (soluble enzyme), 9% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) | Carica papaya |  |
| Ca2+ | 5 mM, 22% loss of activity (soluble enzyme), 6% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) | Carica papaya | |
| Co2+ | 5 mM, 46% loss of activity (soluble enzyme), 30% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) | Carica papaya | |
| Fe2+ | 5 mM, 44% loss of activity (soluble enzyme), 32% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) | Carica papaya | |
| Mg2+ | 5 mM, 19% loss of activity (soluble enzyme), 4% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) | Carica papaya | |
| Mn2+ | 5 mM, 44% loss of activity (soluble enzyme), 31% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) | Carica papaya | |
| additional information | immobilization of papain by covalent attachment on Sepharose 6B activated by using cyanogen bromide brings about resistance against the inhibitory effects of various bivalent metal ions with respect to papain | Carica papaya | |
| Zn2+ | 5 mM, 51% loss of activity (soluble enzyme), 45% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) | Carica papaya | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00062 | - |
casein | soluble enzyme, pH 7.5, 23°C | Carica papaya | |
| 0.00079 | - |
casein | enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide, pH 7.5, 23°C | Carica papaya | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | P00784 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.205 | - |
substrate: casein, enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide, pH 7.5, 23°C | Carica papaya |
| 0.232 | - |
substrate: casein, soluble enzyme, pH 7.5, 23°C | Carica papaya |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Carica papaya | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
soluble enzyme | Carica papaya |
| 80 | - |
enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide | Carica papaya |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life: 18 min (soluble enzyme). Immobilized papain retains 80% of its original activity after 1 h incubation | Carica papaya |
| 90 | - |
half-life: 6 min (soluble enzyme), 40 min (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide). Immobilized papain retains 45% of its original activity after 1 h incubation | Carica papaya |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0102 | - |
casein | enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide, pH 7.5, 23°C | Carica papaya | |
| 0.0162 | - |
casein | soluble enzyme, pH 7.5, 23°C | Carica papaya | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
soluble enzyme | Carica papaya |
| 8 | - |
enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide | Carica papaya |
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