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Literature summary for 3.4.22.2 extracted from
- Characterization of partially folded intermediates of papain in presence of cationic, anionic, and nonionic detergents at low pH (2006), Biopolymers, 83, 1-10.
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| hexadecyltrimethyl ammonium bromide | the presence of hexadecyltrimethyl ammonium bromide allows 41% recovery of enzymatic activity of acid-unfolded papain in the presence of hexadecyltrimethyl ammonium bromide the enzyme exists as a compact intermediate with regain of native-like secondary and partial tertiary structure as well as high 8-anilino-1-naphthalene-sulfonic acid binding with the partially recovered enzymatic activity | Carica papaya |
| SDS | the presence of SDS allows 43% recovery of enzymatic activity of acid-unfolded papain, addition of 8 mM SDS results in the loss of 8-anilino-1-naphthalene-sulfonic acid binding sites exhibited by a decrease in 8-anilino-1-naphthalene-sulfonic acid fluorescence intensity, suggesting the burial of hydrophobic patches, papain at low pH and in the presence of SDS exists in a partially folded state characterized by native-like secondary structure and tertiary folds | Carica papaya |
| Tween | the presence of Tween-20 allows 39% recovery of enzymatic activity of acid-unfolded papain in the presence of Tween-20, acid-unfolded papain exists as a compact intermediate with molten-globule-like characteristics | Carica papaya |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | - |
- |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| acid-unfolded papain in the presence of 8 mM SDS and 3.5 mM hexadecyltrimethyl ammonium bromide retains its partial tertiary structure | Carica papaya |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | - |
Carica papaya | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Carica papaya | ? | - |
? |  |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
the activity of papain is reduced to 35% at pH 2 and in the absence of detergents, at pH 2 papain exhibits a substantial amount of secondary structure and is relatively less denatured compared with 6 M guanidine hydrochloride but loses the persistent tertiary contacts of the native state | Carica papaya |
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