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Literature summary for 3.4.22.2 extracted from

  • Szabelski, M.; Stachowiak, K.; Wiczk, W.
    Influence of organic solvents on papain kinetics (2001), Acta Biochim. Pol., 48, 1197-1201.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
dimethylformamide number of papain active sites decreases with increase of inhibitor concentration Carica papaya
methanol number of papain active sites decreases with increase of inhibitor concentration Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00385
-
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 1% methanol Carica papaya
0.01243
-
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 5% methanol Carica papaya
0.02075
-
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 0.6% dimethylformamide Carica papaya
1.765
-
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 5% dimethylformamide Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans + H2O
-
Carica papaya Dabcyl-Lys-Phe-Gly + Gly-Ala-Ala-Edans
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03 0.55 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 5% methanol Carica papaya
0.41
-
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 0.6% dimethylformamide Carica papaya
2.77
-
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 1% methanol Carica papaya
3 6 Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 1% methanol Carica papaya
6.86
-
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans pH 6.8, 40°C, in presence of 5% methanol Carica papaya