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Literature summary for 3.4.22.16 extracted from

  • Vasiljeva, O.; Dolinar, M.; Turk, V.; Turk, B.
    Recombinant human cathepsin H lacking the mini chain is an endopeptidase (2003), Biochemistry, 42, 13522-13528.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information mutant lacking the mini chain, loss of aminopeptidase activity, increase in endopeptidase activity Homo sapiens
additional information mutant lacking the mini chain, loss of aminopeptidase activity, increase in endopeptidase activity Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
bovine stefin B
-
Homo sapiens
chicken cystatin
-
Homo sapiens
human stefin A
-
Homo sapiens
human stefin B
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
benzoyl-L-Phe-L-Val-L-Arg-4-methylcoumaryl-7-amide pH 6.5, 25°C, native enzyme Sus scrofa
0.038
-
benzoyl-L-Phe-L-Val-L-Arg-4-methylcoumaryl-7-amide pH 6.5, 25°C, mutant lacking the mini chain Sus scrofa
0.082
-
benzoyl-L-Phe-L-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C, mutant lacking the mini chain Sus scrofa
0.1
-
L-Arg-4-methylcoumaryl-7-amide pH 6.5, 25°C, native enzyme Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant protein lacking the mini chain
-
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification mutant lacking the mini chain, autocatalytic processing at pH 5.0, 37°C, processing is accelerated in presence of 0.025 mg/ml of dextran, prolonged activation results in both cases in decrease of enzyme acivtiy Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
Sus scrofa benzoyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
?
benzoyl-L-Phe-L-Val-L-Arg-4-methylcoumaryl-7-amide + H2O
-
Sus scrofa benzoyl-L-Phe-L-Val-L-Arg + 7-amino-4-methylcoumarin
-
?
L-Arg-4-methylcoumaryl-7-amide + H2O
-
Sus scrofa L-Arg + 7-amino-4-methylcoumarin
-
?
Z-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
-
Sus scrofa benzoyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000049
-
chicken cystatin pH 6.5, 25°C, mutant lacking the mini chain Homo sapiens
0.000000074
-
human stefin B pH 6.5, 25°C, mutant lacking the mini chain Homo sapiens
0.000000099
-
human stefin A pH 6.5, 25°C, mutant lacking the mini chain Homo sapiens
0.00000052
-
bovine stefin B pH 6.5, 25°C, native enzyme Homo sapiens