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Literature summary for 3.4.22.15 extracted from

  • Leon-Janampa, N.; Liendo, R.; Gilman, R.H.; Padilla, C.; Garcia, H.H.; Gonzales, A.; Sheen, P.; Pajuelo, M.J.; Zimic, M.; Zimic, M.
    Characterization of a novel cathepsin L-like protease from Taenia solium metacestodes for the immunodiagnosis of porcine cysticercosis (2019), Vet. Parasitol., 267, 9-16 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.15

Application

Application Comment Organism
diagnostics the enzyme is a candidate antigen to diagnose porcine cysticercosis via ELISA immunoassay Taenia solium

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Spodoptera frugiperda SF9 cells using the baculovirus expression system, subcloning in Escherichia coli DH10Bac cells Taenia solium

Inhibitors

Inhibitors Comment Organism Structure
E-64 strong inhibition Taenia solium

Organism

Organism UniProt Comment Textmining
Taenia solium A0A1S5WII4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
metacestode from a naturally infected pig in the central highlands of Peru Taenia solium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Taenia solium benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
Bovine serum albumin + H2O
-
 Taenia solium ?
-
 ?
additional information no activity with human immunoglobulin Taenia solium ?
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin L-like protease
-
 Taenia solium
TsolCL
-
 Taenia solium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Taenia solium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Taenia solium

General Information

General Information Comment Organism
additional information enzyme TsolCL has cathepsin L-conserved amino acid residues in the catalytic site, i.e. Gln8, Cys14, His159, Asn179 and Trp181, and the motif GCNGG. TsolCL is able to distinguish circulating IgG antibodies between healthy animals and naturally infected pigs with cysticercosis Taenia solium