Literature summary for 3.4.22.15 extracted from

de Sousa, L.R.; Wu, H.; Nebo, L.; Fernandes, J.B.; da Silva, M.F.; Kiefer, W.; Schirmeister, T.; Vieira, P.C.
Natural products as inhibitors of recombinant cathepsin L of Leishmania mexicana (2015), Exp. Parasitol., 156, 42-48 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
3-oxo-urs-12-en-28-oic acid	noncompetitive inhibitor	Leishmania mexicana
agathisflavone	noncompetitive inhibitor	Leishmania mexicana
additional information	natural products as inhibitors of recombinant cathepsin L of Leishmania mexicana, overview	Leishmania mexicana
quercetin	-	Leishmania mexicana
tetrahydrorobustaflavone	uncompetitive inhibitor	Leishmania mexicana

Organism

Organism	UniProt	Comment	Textmining
Leishmania mexicana	E9AMH6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O	-	Leishmania mexicana	benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
cathepsin L-like rCPB2.8	-	Leishmania mexicana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Leishmania mexicana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Leishmania mexicana

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	inhibition kinetics	Leishmania mexicana
0.00014	-	agathisflavone	pH 6.5, 25°C	Leishmania mexicana
0.00073	-	tetrahydrorobustaflavone	pH 6.5, 25°C	Leishmania mexicana
0.00126	-	3-oxo-urs-12-en-28-oic acid	pH 6.5, 25°C	Leishmania mexicana
0.006	-	quercetin	pH 6.5, 25°C	Leishmania mexicana

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00043	-	pH 6.5, 25°C	Leishmania mexicana	agathisflavone
0.0022	-	pH 6.5, 25°C	Leishmania mexicana	tetrahydrorobustaflavone
0.00378	-	pH 6.5, 25°C	Leishmania mexicana	3-oxo-urs-12-en-28-oic acid
0.01803	-	pH 6.5, 25°C	Leishmania mexicana	quercetin

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Release 2023.1 (January 2023)