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Literature summary for 3.4.22.15 extracted from

  • Fairhead, M.; Kelly, S.M.; van der Walle, C.F.
    A heparin binding motif on the pro-domain of human procathepsin L mediates zymogen destabilization and activation (2008), Biochem. Biophys. Res. Commun., 366, 862-867.
    View publication on PubMed
Search for more literature for 3.4.22.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X-33 Homo sapiens

Protein Variants

Protein Variants Comment Organism
T223V the mutation removes the potential glycosylation site Homo sapiens
T223V/C138S the mutations prevent catalytic activity Homo sapiens
T223V/C138S/K99A/K104A the mutations disrupt the putative heparin binding site Homo sapiens

General Stability

General Stability Organism
heparin reduces the stability of the pro form of cathepsin L at pH 5 by binding to a putative heparin binding motif (BBXB) in the pro-domain, in contrast heparin only slightly destabilizes the mature cathepsin L domain Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P07711 procathepsin L
-

Purification (Commentary)

Purification (Comment) Organism
heparin-Sepharose chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
procathepsin L + H2O autoactivation Homo sapiens cathepsin L + ?
-
 ?

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 the mature form of cathepsin L exhibits very little activity at pH 8.0 and is largely unfolded Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens calculated value from mature cathepsin L
-
 4.7
Homo sapiens calculated value from procathepsin L
-
 5.3