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Literature summary for 3.4.22.14 extracted from

  • Bublin, M.; Radauer, C.; Knulst, A.; Wagner, S.; Scheiner, O.; Mackie, A.R.; Mills, E.N.; Breiteneder, H.
    Effects of gastrointestinal digestion and heating on the allergenicity of the kiwi allergens Act d 1, actinidin, and Act d 2, a thaumatin-like protein (2008), Mol. Nutr. Food Res., 52, 1130-1139.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Actinidia deliciosa digestibility of kiwifruit allergens, actinidin (Act d 1) and thaumatin-like protein (Act d 2), is assessed using an in vitro digestion system that approximates physiological conditions with respect to the passage of food through the stomach into the duodenum: Act d 1 precipitates in simulated gastric fluid at pH 2 and digestion of the aggregated protein proceeds slowly. The residual precipitate redissolves completely in simulated duodenal fluid at pH 6.5 and is partially digested. Act d 1 and Act d 2 display nearly unchanged IgE binding abilities ?
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Organism

Organism UniProt Comment Textmining
Actinidia deliciosa
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Purification (Commentary)

Purification (Comment) Organism
Act d 1 is purified from kiwifruit extracts by covalent chromatography using activated thiopropyl sepharose 6B Actinidia deliciosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information digestibility of kiwifruit allergens, actinidin (Act d 1) and thaumatin-like protein (Act d 2), is assessed using an in vitro digestion system that approximates physiological conditions with respect to the passage of food through the stomach into the duodenum: Act d 1 precipitates in simulated gastric fluid at pH 2 and digestion of the aggregated protein proceeds slowly. The residual precipitate redissolves completely in simulated duodenal fluid at pH 6.5 and is partially digested. Act d 1 and Act d 2 display nearly unchanged IgE binding abilities Actinidia deliciosa ?
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Synonyms

Synonyms Comment Organism
Act d 1
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Actinidia deliciosa
actinidin
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Actinidia deliciosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
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thermal stability of Act d 1 and Act d 2 is strongly pH dependent. Act d 1 is irreversibly destabilized in acidic solutions (pH 3.5) Actinidia deliciosa