Application | Comment | Organism |
---|---|---|
molecular biology | actinidin compared with type II or IV collagenase isolates intact human umbilical vein endothelial cells, hepatocytes, and thymic epithelial cells with viability more than 90% | Actinidia deliciosa |
synthesis | the enzyme can be used efficiently for hydrolysis of collagen and isolation of different cell populations from various solid tissues | Actinidia deliciosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinidia deliciosa | - |
- |
- |
Actinidia deliciosa | A5HII1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Actinidia deliciosa |
the enzyme fraction is precipitated from kiwifruit extract by 60% saturation of ammonium sulfate. The precipitate is redissolved in 50 mM citrate buffer (pH 5.5) and dialyzed overnight against this buffer. The dialyzate is loaded into a DEAE-Sepharose Fast Flow column, which pre-equilibrated with the same buffer. The adsorbed fractions are eluted with 0.0-1 M linear gradient of sodium chloride in the buffer | Actinidia deliciosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Actinidia deliciosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Collagen type I + H2O | actinidin can hydrolyze collagen types I and II at neutral and alkaline pH | Actinidia deliciosa | ? | - |
? | |
Collagen type I + H2O | the hydrolysis of types I and II collagen by actinidin under different pH conditions is monitored by SDS-PAGE: good digestion of type I collagen at pH 7 (phosphate buffer) or pH 8.5 (Tris-HCl buffer). In citrate buffer (pH 5.5), actinidin digests more than half of the collagen type I, but in acetate buffer (pH 4), the enzyme does not hydrolyze this substrate | Actinidia deliciosa | ? | - |
? | |
collagen type II + H2O | actinidin can hydrolyze collagen types I and II at neutral and alkaline pH | Actinidia deliciosa | ? | - |
? | |
collagen type II + H2O | the hydrolysis of types I and II collagen by actinidin under different pH conditions is monitored by SDS-PAGE: good digestion of type I collagen at pH 7 (phosphate buffer) or pH 8.5 (Tris-HCl buffer). In citrate buffer (pH 5.5), or acetate buffer (pH 4), the enzyme does not hydrolyze this substrate | Actinidia deliciosa | ? | - |
? | |
additional information | actinidin compared with type II or IV collagenase isolated from intact human umbilical vein endothelial cells, hepatocytes, and thymic epithelial cells with viability more than 90% | Actinidia deliciosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
actinidin | - |
Actinidia deliciosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Actinidia deliciosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | efficient digestion of type I and type II collagen | Actinidia deliciosa |