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Literature summary for 3.4.22.10 extracted from

  • Liu, T.Y.; Elliott, S.D.
    Streptococcal proteinase (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 3, 609-647.
No PubMed abstract available
Search for more literature for 3.4.22.10

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol reduction is essential for catalytic activity Streptococcus sp.
thioethanol reduction is essential for catalytic activity, once the enzyme is activated by the reducing agent, the presence of the activator is no longer necessary for catalytic activity Streptococcus sp.
thioglycolic acid reduction is essential for catalytic activity Streptococcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Streptococcus sp.

General Stability

General Stability Organism
0.1 N HCl or 0.01 N NaOH, room temperature, 2 h, denaturation Streptococcus sp.
0.1 N HCl, 2 h, denaturation of the zymogen Streptococcus sp.
0.5% SDS, denaturation Streptococcus sp.
8 M urea plus reducing agent, 20% of proteolytic activity is provided Streptococcus sp.
EDTA, 0.001 M, stabilizes Streptococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrofluorobenzene
-
 Streptococcus sp.
Benzyloxycarbonyl-Gly-Phe competitive against benzyloxycarbonyl-Phe-Tyr Streptococcus sp.
iodoacetamide
-
 Streptococcus sp.
iodoacetic acid
-
 Streptococcus sp.
methyliodine
-
 Streptococcus sp.
NEM
-
 Streptococcus sp.
Sodium tetrathionate
-
 Streptococcus sp.
Tosyl-norleucine-Phe competitive against benzyloxycarbonyl-norleucine-Phe Streptococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effect of pH-value on the Km-value for benzyloxycarbonyl-Lys-p-nitrophenyl ester Streptococcus sp.
0.22
-
 benzyloxycarbonyl-Lys-p-nitrophenyl ester pH 4.27 Streptococcus sp.
0.262
-
 benzyloxycarbonyl-Lys-p-nitrophenyl ester pH 6.35 Streptococcus sp.
0.494
-
 benzyloxycarbonyl-Lys-p-nitrophenyl ester pH 4.49 Streptococcus sp.
2.28
-
 benzyloxycarbonyl-Lys-Phe
-
 Streptococcus sp.
2.64
-
 Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe
-
 Streptococcus sp.
3.88
-
 benzyloxycarbonyl-Glu-Phe
-
 Streptococcus sp.
5.05
-
 Nalpha-benzyloxycarbonyl-Lys-Phe
-
 Streptococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
bisulfite reduction is essential for catalytic activity Streptococcus sp.
cyanide reduction is essential for catalytic activity Streptococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 high-speed equilibrium ultracentrifugation Streptococcus sp.
33590
-
 determination of amino acid composition Streptococcus sp.

Organism

Organism UniProt Comment Textmining
Streptococcus sp.
-
 hemolytic
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification
-
 Streptococcus sp.

Renatured (Commentary)

Renatured (Comment) Organism
90% renaturation provided the denatured zymogen is solubilized with urea or guanidinium chloride and given time to refold Streptococcus sp.

Storage Stability

Storage Stability Organism
-20°C, stable for at least 2 years Streptococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Glu-p-nitrophenyl ester + H2O
-
 Streptococcus sp. ?
-
 ?
benzyloxycarbonyl-Glu-Phe + H2O
-
 Streptococcus sp. ?
-
 ?
benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
-
 Streptococcus sp. benzyloxycarbonyl-Gly + p-nitrophenol
-
 ?
benzyloxycarbonyl-Nle-p-nitrophenyl ester + H2O
-
 Streptococcus sp. ?
-
 ?
benzyloxycarbonyl-Nle-Phe + H2O
-
 Streptococcus sp. ?
-
 ?
N-benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
-
 Streptococcus sp. N-benzyloxycarbonyl-Gly + 4-nitrophenol
-
 ?
Nalpha-benzyloxycarbonyl-Lys-Phe + H2O
-
 Streptococcus sp. ?
-
 ?
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe + H2O
-
 Streptococcus sp. ?
-
 ?
Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys-p-nitrophenyl ester + H2O
-
 Streptococcus sp. Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys + p-nitrophenol
-
 ?
Nalpha-benzyloxycarbonyl-Phe-Tyr + H2O
-
 Streptococcus sp. ?
-
 ?
Nepsilon-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O
-
 Streptococcus sp. Nepsilon-benzyloxycarbonyl-Lys + 4-nitrophenol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 0.1 N HCl, 2 h, denaturation of the zymogen Streptococcus sp.
60
-
 irreversible inactivation Streptococcus sp.
100
-
 pH 6.7, 10 min, denaturation of the zymogen Streptococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information effect of pH-value on the turnover numer for benzyloxycarbonyl-Lys-p-nitrophenyl ester Streptococcus sp.
1.09
-
 benzyloxycarbonyl-Lys-Phe
-
 Streptococcus sp.
2.3
-
 benzyloxycarbonyl-Glu-Phe
-
 Streptococcus sp.
5.05
-
 Nalpha-benzyloxycarbonyl-Lys-Phe
-
 Streptococcus sp.
8.6
-
 Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe
-
 Streptococcus sp.
29.7
-
 benzyloxycarbonyl-Lys-p-nitrophenyl ester pH 4.27 Streptococcus sp.
89.8
-
 benzyloxycarbonyl-Lys-p-nitrophenyl ester pH 4.49 Streptococcus sp.
142
-
 benzyloxycarbonyl-Lys-p-nitrophenyl ester pH 6.35 Streptococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9.5 unstable below pH 4.5 and above pH 9.5 Streptococcus sp.