Any feedback?
Literature summary for 3.4.22.10 extracted from
- Metal-mediated modulation of streptococcal cysteine protease activity and its biological implications (2014), Infect. Immun., 82, 2992-3001.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Streptococcus pyogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | Cu2+ inhibits the proteolytic activity of mature SpeB | Streptococcus pyogenes |  |
| N-(N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucyl)-agmatine | - |
Streptococcus pyogenes | |
| Zn2+ | Zinc inhibits the proteolytic activity of mature SpeB | Streptococcus pyogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
mature SpeB | Streptococcus pyogenes |
| 42000 | - |
enzymatically inactive zymogen form of SpeB | Streptococcus pyogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| streptococcal mitogenic exotoxin Z + H2O | Streptococcus pyogenes | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pyogenes | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | Spe is expressed as a propeptide. Zinc blocks the maturation of the SpeB zymogen and thereby the proteolytic degradation of proteins by SpeB | Streptococcus pyogenes |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Streptococcus pyogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| streptococcal mitogenic exotoxin Z + H2O | - |
Streptococcus pyogenes | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SpeB | - |
Streptococcus pyogenes |
| streptococcal pyrogenic exotoxin B | - |
Streptococcus pyogenes |
| streptopain | - |
Streptococcus pyogenes |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0279 | - |
pH and temperature not specified in the publication | Streptococcus pyogenes | Zn2+ | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
