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Literature summary for 3.4.22.10 extracted from

  • Tsao, N.; Tsai, W.H.; Lin, Y.S.; Chuang, W.J.; Wang, C.H.; Kuo, C.F.
    Streptococcal pyrogenic exotoxin B cleaves properdin and inhibits complement-mediated opsonophagocytosis (2006), Biochem. Biophys. Res. Commun., 339, 779-784.
    View publication on PubMed
Search for more literature for 3.4.22.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant C192S enzymes in Escherichia coli strain BL21(DE3) Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
C192S inactive mutant Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, mature enzyme, SDS-PAGE Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
properdin + H2O Streptococcus pyogenes cleavage of properdin leads to inhibition of complement-mediated opsonophagocytosis and bacterial killing by neutrophils, alternative complement pathway overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme performs autolytic processing to the mature 28 kDa protein by removal of an about 117 amino-acid peptide from the N-terminus Streptococcus pyogenes

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant C192S enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the wild-type enzyme autoprocesses during purification to the mature 28 kDa protein Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
properdin + H2O
-
 Streptococcus pyogenes ?
-
 ?
properdin + H2O cleavage of properdin leads to inhibition of complement-mediated opsonophagocytosis and bacterial killing by neutrophils, alternative complement pathway overview Streptococcus pyogenes ?
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, mature enzyme, SDS-PAGE Streptococcus pyogenes

Synonyms

Synonyms Comment Organism
Spe B
-
 Streptococcus pyogenes
streptococcal pyrogenic exotoxin B
-
 Streptococcus pyogenes